Gene Transfection In Non Target Tissue When The Ultrasound Mediated Exogenous SDF-1? Gene Transfer To The Heart In Rats With Acute Myocardial Infarction

Objective: To evaluate gene transfection in liver,lung and kidney when the ultrasound,microbubble and recombinant adenovirus mediated exogenous stromal cellderived factor-1?(SDF-1?)gene transfer to the heart in rats with acute myocardial infarction(AMI).Method: According to the method for querying random number table,all 40 AMI SD rats were randomly divided into control and experimental groups:myocardial infarction + ultrasound irradiation group(M+U/ Control group,n=10);myocardial infarction + ultrasound irradiation+ carrying pAd-EGFP/SDF-1?(The biotin recombinant adenovirus expressing enhanced green fluorescent protein and SDF-1.)gene-microbubble administration 1 day group(M+S1+U,n=10),continuous administration two days group(M+S2+U,n=10),continuous administration three days group(M+S2+U,n=10).The expression of EGFP in liver,lung and kidney were detected by laser scanning confocal microscopy at seven days after administration.IPP6.0 software was used to detecte the expression area of EGFP.Result: Expression of EGFP in lung,liver and kidney(% area): M+U: 0,0,0;M+S1+U: 2.57±0.88,2.31±0.77,2.30±0.80;M+S2+U: 2.97±0.94,2.89±1.00,2.77±0.82;M+S3+U: 3.01±0.71,2.98±0.86,2.89±0.79.The difference between all the gene delivery groups and the control group was statistically significant(P < 0.05),With the increase of the number of medication days,the transfection increased slightly,but there was no significant difference between the different drug delivery groups.Conclusion: When the ultrasound,microbubble and recombinant adenovirus mediated exogenous SDF-1? gene transfer to the heart in AMI rats,liver,lung and kidney tissues will also be transfected.However,with the increase of the days of administration,the transfection of target gene in non-target tissue produced only a slight accumulation.The transfection area of target gene in non-target tissue was not linear correlated with the days of administration.Transfection of target gene in non target tissue did not produce significant cumulative effect.