The Expression And Its Relationship With Clinical Stage And Differentiation Of Tenascin C In Rectal Cancer And Gastric Cancer

Background and purposeWith the aging of our population and the changes in the natural environment,malignant tumors have become one of the main causes of death.The results of the national health sector show that the first cause of the death of urban population in China is malignancy,and the main type of malignancy is cancer.In addition,the statistical results show that,on a global scale,rectal cancer Colorectal carcinoma,CRC)is the second most common type of tumor in women,and that rectal cancer is the third most common type of tumor in men.Gastric cancer(GC)ranks second in global tumor death.n recent years,the incidence of rectal cancer and gastric cancer increased year by year,and for the treatment of rectal cancer and gastric cancer is mainly surgical and postoperative chemotherapy conservative treatment,some patients will be recurrence,metastasis and chemotherapy tolerance and other sequelae.Tendonin C,also known as cell line caffeine(Tenascin C,TN?C),is one of the new extracellular matrix(ECM)components found in recent years.It is mainly involved in embryogenesis,tumorigenesis,proliferation and its Metastasis,tissue repair after trauma and fibrosis and cell aging process.It was found that TN?C was mainly involved in tumor growth,metastasis,angiogenesis and immunosuppression,and TN?C was highly expressed in malignant tumors such as breast cancer and kidney cancer,which was related to tumor invasion and metastasis and its prognosis.This study was designed to observe the expression of TN?C in rectal cancer and gastric cancer in digestive tract tumors and its clinical significance.Method 1.Immunohistochemical technique was used to treat 34 specimens of rectal cancerand 5 patients with synchronous polyps in the 105 th Hospital of People'sLiberation Army.The changes of tendon protein C(Tenascin C,TN?C)in rectalcancer tissues Differences in the expression of specimens from specimens andintestinal polyps.2.Immunohistochemical technique was used to treat 29 cases of gastric cancer and5 cases of patients with gastric ulcer of the same period.The changes of tendonprotein C(Tenascin C,TN?C)in gastric cancer tissues and gastric ulcer Differencesin the expression of tissue samples.3.All the 34 specimens of rectal cancer and 5 patients with synchronous polypswere treated with fluorescence quantitative PCR.The expression of tenascin C(TN?C)gene in gastric cancer tissue And gastric ulcer tissue samples of theexpression differences.4.By fluorescence quantitative PCR technology,respectively,the 105 th Hospital ofPeople's Liberation Army patients were sent to 29 cases of gastric cancer and 5cases.The expression of Tenascin C(TN?C)gene in gastric cancer tissue and gastriculcer tissue was compared with that of gastric ulcer tissue.5.To analyze the expression of tendon protein C in different degrees ofdifferentiation and clinical stage of rectal cancer.6.To analyze the expression of tendon protein C in different degrees ofdifferentiation and clinical stage of gastric cancer.Result 1.Rectal cancer patients with the same period of intestinal polyps in patients withtissue samples,rectal cancer specimens tendon?C expression was positive.2.The expression of tendon?C in gastric cancer specimens was positively correlatedwith gastric tissue in patients with gastric ulcer.3.The expression of tendon?C gene in rectal cancer specimens was significantlyhigher than that in colorectal polyps patients.4.The expression of tendon?C gene in gastric cancer specimens was significantlyhigher than that in gastric ulcer patients.5.The expression of TN?C was significantly increased with the increase of histologicalgrade of rectal cancer.The difference was statistically significant(P <0.05)in theexpression of TN?C in well differentiated and moderately differentiated andmoderately differentiated carcinogenic tissues.(P <0.05).The expression of TN?Cwas also increased with the increase of clinical stage.The expression of TN?C instage ? ~ ? rectal cancer was significantly higher than that in stage ? ~ ?(P<0.05).The expression of TN?C was significantly increased with the increase of pathologic grade of gastric cancer.The difference was statistically significant(P <0.05).6.The expression of TN?C was significantly different between well differentiated and moderately differentiated and moderately differentiated carcinogenic tissues.The expression of TN?C was also increased with the increase of clinical stage.The expression of TN?C in patients with stage ? ~ ? gastric cancer was significantly higher than that in patients with stage ? ~ ?(P <0.05).ConclusionThe expression of tendon protein C in rectal cancer and gastric cancer tissues is related to the degree of tumor differentiation and clinical stage.