Knockdown Of Long Noncoding RNA ATB Inhibits The Proliferation,Migration And Invasion Of Glioma Cells

Objective To investigate the effects of Long non-coding RNA activated by TGF-?(ATB)on the proliferation,migration and invasion of human U87 glioma cell line.Methods The expression of ATB in human U87 glioma cell line and human normal astrocyte(HEB)were detected by real-time quantitative PCR(q RT-PCR).And based on this,we constructed sh RNA ATB plasmid,which could knockdown ATB expression after transfected with U87 cells.The proliferation ability of cell was investigated through MTT Assay and the colony-formation ability was tested by the cell cloning Assay;Cell migration and invasion were measured by non-Matrigel transwell Assay and Matrigel transwell Assay in vitro.Western blot was performed to measure the PNCA protein expression levels.Results Using qRT-PCR analysis,we found ATB expression levels in U87 cell line was significantly increased compared with HEB(P<0.01);The expression of ATB was remarkable reduced after U87 transfected with sh RNA-ATB plasmid(the experimental group)compared with U87 cells transfected with sh RNA control(the control group);Cloning formation experiment indicated that colony-formation ability is weaker than that of control group(P<0.01);MTT Assay indicated that the number of viable cell was lower than that of control group(P<0.01).Transwell Assay further verified that knockdown of ATB significantly inhibited the migration and invasion ability of U87 cells(P<0.01).In addition,Western blot indicated that the PCNA protein were decreased in the sh RNA-ATB group compare to the sh RNA-control group,which suggested that sh RNA-ATB group had fewer proliferative cells than that in the sh RNA-control group.Conclusion ATB knockdown inhibit glioma cell proliferation,migration and invasion,which indicated ATB may represent a potential therapeutic target for the treatment of human glioma.