Effects And Mechanism Of High Nutritional Status On Morphology And Tumorigenicity Of Tumor Cells

The role of nutritional status in cancer treatment has been the subject of much research.As such,therapies which aimed at tumor nutritional environment and energy metabolism offer a broad clinical prospect.Instead of “starving tumors”,our aim was to explore the effects of high nutritional status on morphology and Tumorigenicity of tumor cells and explore the possible mechanism.Tumor cells were cultured supplemented with varying concentrations of fetal bovine serum to simulate different nutritional status(1%,2.5%,5%,10%,25% and50%).After 3 weeks,Cell proliferation was assessed by Cell Counting Kit-8,then tumorigenic and metastatic capacity were evaluated by colony formation in vitro,Xenograft Tumorigenicity assay in vivo,adhesion assay,migration assay and Western blotting analysis.Oil red O staining,flow cytometry,electron microscopy,and Western blotting analysis were used to investigate the possible mechanism.It was found that high High FBS concentrations can promote the proliferation and vacuolization of tumor cells,while normal cells remained unaffected.Moreover,High FBS concentrations blunt colony formation in vitro and inhibit tumorigenicity in vivo,inhibit tumor metastasis and reduce the degree of malignancy.Conversion of Light Chain 3 and observation of autophagic vacuoles in treated PC3 M cells,together with no significant changes in Oil red O staining and apoptosis,indicate that excess nutrition are likely to have a specific role in inhibiting tumorigenicity through an autophagic pathway.Based on the inhibition of tumorigenic and metastatic capacity of tumor cells,we propose that high nutritional status has potential as a therapeutic strategy for cancers.