The Significance Of Combined Detection Of Pathogen Antibody And Inflammatory Markers In Children With Respiratory Tract Infections

Background and objectiveRespiratory disease is apt to contagious in children,due to the multiple and complicated causes of infection,it is easy to cause severe or systemic complications.However,owing to the pathogen is not to identify,children cannot express their symptoms in clearly and the other reasons.It is the reason that children's respiratory tract infection is difficult to detect.Doctors often write out a prescription for patients on the condition of the pathogen in ambiguity.The consequence is that pathogen coexists with together more and more and abuse of antibiotics.It brings a great difficult to clinical treatment.This study is that to find a better detection method for the children respiratory tract infection.Based on the nine kinds of IgM in detection and coexists with the other inflammatory markers to detect.According as the statistical results of the main pathogens of respiratory tract infection in children in our area,and find the best method of detection of pathogens in children with respiratory tract infection.It is aimed that give accurately provide diagnosis basis for the treatment of respiratory infection in children.MethodsFirstly,collection the 3578 children`s blood that who suffer from respiratorytract infection in Luohe Centra Hospital,during the period from September 2013 to Aguest 2015.The study used the indirect immunofluorescence(ILF)method and IgM antibody reagents to detect Legionella pneumophlia 1(LP1),Mycoplasma pneumoniae(MP),Coxiella burnetii(COX),Chlamydia pneumoniae(CP),Adenovirus(ADV),Respiratory syncytial virus(RSV),Influenza A virus(IFA),Influenza B virus(IFB),Parainfluenza virus(PIV).And then select one of the 305 cases of pneumonia mycoplasma detection of IgM antibody positive.The patient`s aged from 0 to 14 years old.The study that analysis the bacterial infection in children used the double antibody labeling,detected the serum procalcitonin by chemiluminescence method and detection of the CRP by immunoturbidimetric assay,and combined with the results of WBC counting.The data were analyzed by SPSS18.0 data analysis,using the standard deviation((?)±s)said that the association of r>0.3,P<0.05 was statistically significant.Results(1)There is a 1258 cases(35.15%)IgM antibody positive was detected in 3578 children.And the detection rate in descending order MP,FluB,PIV etc,484 cases of mixed infection and it`s 13.52% among all mixed infection.Positive infection of men were 18.89% and women were 16.26% among all infective sufferers.There was a highest positive rate was 13.86% that is the 1-3 years old group.The second is 3-5years old group(8.16%).There was a significant difference(P<0.05)between the groups.(2)We have analyzed that seasonal viral infection in children,The result is that winter(14.08%)and spring(8.30%)higher incidence.(3)The results about the nine kinds of IgM in detection that inflammatory markers were detected in 31.14% of the children.It is said that there is a undetected in pathogen antibody detection.Because of PCT was higher(30.49%).Therefore,there was a increased to detection rate of bacterial infection used the combined detection.(4)The study make an analysis of bacterial infections in infant group and preschool group with mycoplasma pneumonia.There was a significant difference between infant group and preschool children group(P<0.05).ConclusionThe majority of the nine kinds of IgM in detection in children is MP in our area.There are seasonal,gender and age differences.It is a improve of the detection rate of Mycoplasma pneumoniae infection complicated with bacterial infection with the nine kinds of Ig M in detection coexists with PCT,CRP and WBC.It is clear that can provide more accurate evidence for clinical diagonsis.