Studies On Preparation And Pharmacokinetic Of Ginkgo Biloba Extract Cationic Liposomes

To improve the oral bioavailability of the ginko biloba extract,we established the Ethanol injection method for the preparation of liposome solution dispersion of EGB.As the un-stability of liposome dispersion,such as easy oxidation,aggregation,hydrolyzation and drug release,we prepared the new drug delivery system pro-liposome,adding chitosan to form cationic liposomes to increase the absorption of intestinal.We added the carrier material to the liposome solution dispersion,using the spry dryer to prepare the pro-liposome powder.The EGB's pro-liposome drug delivery system improved the stability of liposome,and possessed a good morphology and encapsulation efficiency afer hydration.The EGB's pro-liposome drug delivery system may promote the wide application for oral drug.In this article,we firstly established the vitro analysis of ginkgo biloba flavonal glycosides and terpene lactones using the HPLC method.We research the stability,accuracy,specificity of the method and built the standard curve with a good linearity.After establishing the method,we studied the preparation of EGB's pro-liposome.We investigated the factor of the liposome solution dispersion affecting the particle size and encapsulation efficiency.The factor included the prepared temperature,stirring rate,two-phase volume ratio,reaction time.We also researched the inlet temperature and speed,carrier type in the process of sprying.Lastly,we conducted the formulation optimization using central composite design.The confirmatory experiment for the 3 batches of samples got the good encapsulation efficiency(87.28%?86.86%,85.98%)in according with theory results.We established analysis method of ginkgo biloba flavonal glycosides and terpene lactones using the HPLC-MS/MS in vivo,and studied the pharmacokinetic of EGBN.