Coreopsis Basalis Active Components On Pancreatic Islet Cell Protection And Small Molecules Knock Out Preliminary Exploration

Objective: To investigate the protective effect of 1 flavonoids of Coreopsis basalis MAREIN on pancreatic beta cells and the possible mechanism;effect of 2 chrysanthemum polysaccharides on proliferation and insulin secretion of pancreatic beta cells;antigen synthesis and identification of artificial 3 MAREIN analogues for the preparation of naringin,naringin the monoclonal antibody and establish corresponding immunoassay for small molecular knockout naringin foundation.Methods: 1 using four methyl thiazolyl tetrazolium colorimetric assay(MTT)to detect its activity;enzyme linked immunosorbent assay(ELISA)detection of insulin secretion;Western Blot method for detection of BAX in cells,the expression level of Bcl-2 protein;2 using four methyl thiazolyl tetrazolium colorimetric assay(MTT)to detect its activity;ELISA method(ELISA)detection of insulin secretion;3 by periodate oxidation synthesis of naringin artificial antigen;UV and TLC identification of artificial antigen coupling success.Results: 1 compared with the normal control group,the cell survival rate of high glucose and high fat group was significantly lower(P <0.05),and the proliferation of MIN6 cells was significantly increased in the treatment group(P<0.05).Compared with the normal control group,the insulin secretion in the high glucose and high fat group(P <0.05)was significantly lower than that in the model group with high glucose and high fat,and the insulin secretion in the treatment group was significantly higher(P <0.05).Western blot showed that,compared with the normal control group,the expression of BAX protein in model group increased,with the increasing of the concentration of the drug group decreased;Bcl-2 protein in model group decreased,the drug group increased with the increase of concentration(P<0.05;2)compared with the normal control group,high-fat model group cell survival rate decreased significantly(P <0.05),different concentrations of chrysanthemum polysaccharide on the proliferation of MIN6 cell line were significantly increased(P<0.05).Compared with the normal control group,high-fat model group cell insulin secretion decreased significantly(P <0.05);compared with the high-fat model group,drug group to insulin secretion increased significantly(P <0.05 3);UV spectra showed that artificial antigen was successful;TLC showed that the artificial antigen coupling success.Conclusion: 1 MAREIN caused by high glucose and lipid on the islet cells injury has a protective effect,its mechanism may be related to the Bcl protein family;2 chrysanthemum polysaccharides can reverse the damage glucolipotoxicity on islet cells;naringin artificial antigen was synthesized successfully in 3,and small molecules can be prepared next pummelo peel glucoside monoclonal a knocking out method of antibody for.