Differential Expression And Bioinformatics Analysis Of LncRNA In Pemphigus Vulgaris

Pemphigus vulgaris(PV)is a common type of chronic bullous skin disease,the cause of which has not yet been clear,is usually considered an autoimmune response [1].The current treatment of its use of glucocorticoids,long-term use of side effects or even abuse can be life-threatening.Therefore,it is urgent to explore the pathogenesis of PV and to find potential key biological regulation sites.Long-stranded non-coding RNA(LncRNA)has been shown to regulate gene expression in epigenetic,transcriptional and post-transcriptional levels,and is closely linked to the development and progression of many diseases.There are no reports about LncRNA and PV research at home and abroad.Objective:In this study,the differential expression of LncRNA and mRNA in PBMC of PV patients was detected by gene chip technique,and preliminary bioinformatics analysis was carried out.Methods: The differential expression of LncRNA and mRNA in PV patients and normal PBMCs was screened by gene chip technique.The expression of LncRNA was predicted by q RT-PCR.The expression of LncRNA was predicted by indirect expression of LncRNA.The results showed that the expression of LncRNA target gene and differentially expressed mRNA were compared and analyzed to find out the possible role of PV in the development of PV,and the expression of LncRNA target gene and differentially expressed mRNA was analyzed by GO and KEGG enrichment analysis.Mechanism or signaling pathway,lay the foundation for subsequent LncRNA functional validation.Results:A total of 40343 LncRNAs were found,among which the expression of LncRNA was 219,up to 142%,accounting for 0.35198% of the total.The number of LncRNA was 789,accounting for 0.19086% of the total.21422 mRNAs were found,of which 74 were differentially expressed 34,accounting for 0.15871% of the total;down 40,accounting for 0.18672% of the total;Four of the LncRNAs were selected for q RT-PCR,and the results were consistent with the results of gene chip test,which proved that the chip detection was accurate and reliable.A total of 1484 target genes were found,including 137 cis target genes and 1347 Trans target genes.The resulting target genes are mainly enriched in the following biological processes: p53 signaling pathway / endocytosis / lysosome / valine,leucine and isoleucine degradation / apoptosis / tryptophan metabolism / fatty acid degradation / Ribosome / RNA transport / Toll-like receptor signaling pathway / T cell receptor signaling pathway / prostate cancer / peroxisome / endometrial cancer / chronic myeloid leukemia / B cell receptor signaling pathway / acute myeloid leukemia Wait;The differential expression of mRNA is mainly involved in the regulation of peroxisome proliferator-activated receptor signaling,regulation of chemokine secretion,regulation of tolerance tolerance,heparin biosynthesis and metabolic processes such as biological processes and ribosomes,Jak-STAT signaling pathway,hematopoietic cell lineage,cytokine receptor interaction and other signaling pathways.The three mRNAs in the main pathway were identified:RPL21,IDO1,CCR3,the target gene of LncRNA and the differentially expressed mRNA.Conclusion: Compared with normal controls,there were differences in the expression of LncRNA in peripheral blood PBMCs,and q RT-PCR further validated the accuracy of the chip results.Abnormal expression of LncRNA may play an important role in the development of PV.These LncRNAs are likely to regulate the target gene through Cis or Trans,which in turn affects the occurrence and development of ribosomes,Jak-STAT signaling pathway,hematopoietic cell lineage and cytokine receptor interactions.