Effect And Mechanism Of Ap0lipoprotein E On The Expression Of Inflammatory Cytokines And Matrix Metalloproteinases-9 In Astrocytes

Objective To effect and mechanism of apolipoprotein E on the expression of inflammatory cytokines and matrix metalloproteinases-9 in astrocytes.Methods Separated to Group Activation,Antibody Group and Control Group randomly is the methods for vitro cultivate of ApoE-/-in C57 BL / 6 and the primary astrocytes in wild type(WT)mice.Astrocytes were identified by antibodies against Anti astrocyte acidic protein(GFAP),and detected by anti MMP-9 antibody to determine the qualitative expression of MMP-9.Separated to Control Group,Antibody Group and Group Activation randomly is the methods for vitro cultivate of the primary astrocytes in ApoE-/-and WT.Control group received no intervention,the addition of the pertussis toxin and the heat-inactivated tuberculin H37 Ra stimulate in Activation Group.On the basis of the activation group,the antibody group is divided into 4 types(the antibody group+ anti TNF-alpha?the antibody group+ anti IL-12?the antibody group+ anti IL-6?the antibody group+ anti IFN-gamma),respectively,adding anti TNF-alpha,IL-12,IL-6,IFN-gamma antibody to inhibit the inflammation due to the corresponding.ELISA method was used to detect the concentration of MMP-9 and TIMP-1 in each group to determine the change of MMP-9 andTIMP-1 concentration in the activation group compared with the control group and the antibody group;Then,the concentrations of TNF-alpha,IL-12,IL-6,and IFN-gamma inflammatory factors in three groups were detected,in addition,according to the addition of different antibodies,the antibody group only detected the corresponding inflammatory factors.Result ApoE-/-and WT astrocytes were qualitative expression of MMP-9.Compared with the control group,ApoE-/-and WT astrocyte activation group relative to the concentration of MMP-9 in the activated group was significantly higher than that in the control group(P<0.05),the kinds situation of the antibody group was significantly lower than that of the active group(P<0.05),while there was no significant difference in the concentration of TIMP-1 in astrocytes of ApoE-/-and WT,and the difference was not statistically significant(P>0.05).Compared with the control group,the concentrations of inflammatory cytokines in the activated group of ApoE-/-and WT were significantly higher than those in control group(P<0.05),after the addition of antibody to the four cases,the concentration of inflammatory factors in the antibody group was significantly lower than that in the activated group,and the difference was statistically significant(P<0.05),The concentration of inflammatory factors in ApoE-/-was higher than that in WT.Conclusion ApoE can regulate the expression of MMP-9 by regulating the secretion of proinflammatory cytokines,which can affect the integrity of the blood brain barrier.The relationship ApoE,inflammatory factors and MMP-9may serve as a new starting point for the prevention and treatment of demyelinating diseases of the central nervous system.