Glassy Carbon Electrode Modified With G-MoS₂-Nafion As Electrochemical Biosensor Determines Uric Acid In Human Serum

Objectives: At present,most of the methods used for detection of uric acid(UA)can not reach the requirements of fast and accurate,high sensitivity,high specificity,wide linear range,low cost,etc.The detection results of others biological or chemical components of nature indicates that electrochemical methods is possible to satisfy above-mentioned.But the main difficulty of this method is disscover an ideal electrode material to detected the certain component.In this study,our aim was to find the ideal electrochemical sensing material with high sensitivity,fast detection and wide linear range for the detection of UA.Methods: We developed a glassy carbon electrode(GCE)modified with graphene-Mo S2-Nafion(G-MoS2-Nafion)composites as the working electrode.The morphologies and elemental compositions of the G-MoS2 composites were characterized by field emission scanning electron microscopy(SEM),elemental distribution spectrometry(EDS)and X-ray diffraction(XRD),respectively.The electrochemical behaviors were investigated by cyclic voltammetry(CV),linear sweep voltammetry(LSV)and the amperometric i-t curve(i-t).Then the interference of glucose,ascorbic acid and dopamine,and the accuracy and precision of electrochemical method were evaluated.Results: Our study identified the following:(1)the sigle reduction peak of UA was detected in human serum samples indicated that the methodestablished in this study had high specificity for the determination of UA in human serum;(2)UA concentration has a linear relationship with the current intensity(y=0.012x+0.998,R2=0.998).And this method has wide linear range and high sensitivity(minimum detectability 3S/N =13.91?M)for the UA detection;(3)the values of UA in human serum determined by electrochemical method and uricase colorimetric assay were positively relative(y=0.9802x+11.494,R2=0.978);(4)the average recovery rate of UA is 95.2%,indicated that this method had high accuracy;(5)the replicability assay of i-t electrochemical method showed that the method had a good precision for the UA detection.Conclusions: Our study vertified that glassy carbon electrode modified with graphene-Mo S2-Nafion had high sensitivity,wide linear range,fast detection,good anti-interference ability,low cost,good accuracy and replicability in determing serum UA.We believe that G-MoS2-Nafion has a good clinical application prospect for the detection of the concentration of UA in human serum.