Effects Of Sevoflurane Postconditioning On Oxidative Stress And Inflammatory Reaction During Cerebral Ischemia-reperfusion In Rats

Objective:To investigate the effect of sevoflurane postconditioning on oxidative stress and inflammatory reaction during cerebral ischemia-reperfusion in rats,and to explore its cerebral protective mechanism.Methods:Thirty-six health male Sprague-Dawley rats(weighing 220-260 g,12-14 weeks),were randomly assigned into 3 groups(n=12):sham operation group(group Sham),cerebral ischemia-reperfusion group(group I/R)and sevoflurane postconditioning group(group SPC).Cerebral ischemia-reperfusion model was produced by middle cerebral artery occlusion,ischemia 30 min followed by reperfusion 24 h in group I/R and group SPC.In group SPC,2.6% sevoflurane was inhaled for 15 min at the beginning of reperfusion.At 24 h of reperfusion,using the Longa method to recording neurobehavioral score,rats were sacrificed and brain tissue was taken out.The cerebral infarct size was measured by TTC staining,Iba-1 and HO-1 expressions were measured by Western blot,The levels of ROS,SOD,MDA,TNF-? and IL-1? were measured.Results:(1)Compared with group Sham,the neurobehavioral score and cerebral infarct size were increased in group I/R and SPC(P<0.05),Compared with group I/R,the neurobehavioral score and cerebral infarct size were decreased in group SPC.(2)Compared with group Sham,Iba-1 expression was up-regluated,MDA,TNF-?,IL-1? and ROS levels were increased,but SOD level was decreased,HO-1 expression was down-regluated in group IR and SPC(P<0.05),Compared with group I/R,Iba-1 expression was down-regluated,MDA,TNF-?,IL-1? and ROS levels were decreased,but SOD level was increased,HO-1 expression was up-regluated in group SPC(P<0.05).Conclusion:Sevoflurane postconditioning can mitigate the microglia activation,reduce cerebral oxidative stress and inflammation reaction,further protect against cerebral ischemia reperfusion injury in rats.