Multiparameter Flow Cytometry In Childhood Myelodysplastic Syndromes

Objective:A retrospective analysis of 42 childhood MDS was conducted to value the importance of flow cytometry and flow cytometric scoring system(FCSS).Methods:A retrospective analysis was performed on 42 pediatric patients diagnosed with MDS from 2014 May to 2016 August on basis of pediatric MDS criterion.Mutiparameter flow cytometry was performed to investigate the immunophenotyping features and populations of each lineage.Results:Following abnormal antigen expressions were found in the samples:increased percentage of abnormal myeloid progenitor cells(48%),increased lymphoid to myeloid ratio(>1)(45%),presence of lymphoid antigens on myeloid subpopulations(33%),abnormal relationship between CD13 and CD16 in granulocytes(19%)?presence of CD56 on monocytes or myeloid subpopulations(14%),abnormal granularity(7%),lack of CD33 or CD11b expression(4%),lack of CD 14 expression on monocytes(2%),lack of CD71 or CD36 in erythroid precursors(12%).The median overall surviveal time of patiens in FCSS subgroups was:25 months(normal/mild),13 months(moderate),11 months(severe)(P=0.013),and the median lueakemia free surviveal time were respectively 24,13,8 months(P=0.000).Significant correlation was found between the FCSS and MDS claasification,IPSS,IPSS-R(Spearman r respectively was 0.674?0.604?0.693;P<0.005).Conclusion:Flow cytometry is of guiding significance in diagnosis and prognosis evaluation of childhood MDS.As a quantitative scoring system,further research is sill needed to evaluate the sensitivity and specificity of flow cytometric analysis in pediatric MDS.