TMT Technology-based Differences Of Protein Expression In Macrophages Infected By Mycobacterium Tuberculosis And Nontuberculous Mycobacterium

Objective To screen high specificity and sensitivity differential proteins,the macrophages were respectively infected by mycobacterium tuberculosis and nontuberculous mycobacterium.The study was expected to provide evidence for identification of MTB and NTM.Methods The macrophages were respectively infected by mycobacterium tuberculosis and nontuberculous mycobacterium for the MTB infection model and the NTM infection model;extracting the total proteins in the cell;TMT technology was used to detect differential proteins.Results With TMT technology, the differential expression proteins were detected by mycobacterium tuberculosis and nontuberculous mycobacterium infecting the macrophages.887 proteins are showed significantly differences in the MTB infection group; 574 proteins are showed significantly differences in the NTM infection group;682 proteins are showed significantly differences in the MTB infection group not NTM infection group; 369 proteins are showed significantly differences in the NTM infection group not the MTB infection group;205 proteins are showed significantly differences in both the NTM infection group and the MTB infection group.Conclusions The study has detected differential proteins in mycobacteriumtuberculosis infection group and nontuberculous mycobacterium infection group using TMT technology.The results show that part of the differential proteins both in the TMT and NTM infection groups,indicating that MTB and NTM have similar pathogenic factors; and part of the differential proteins only in the MTB infection group or in the NTM infection group,indication that MTB and NTM have differential pathogenic factors.