IL-17 Suppresses Infection Of Candida Albicans In Human Oral Mucosal Epithelium Cells:A Vitro Study

Objective: This study was to investigate the effects of IL-17 A on expression of human beta-defensins(h BDs),S100A8,LL-37 cytokines of oral mucosal epithelial cells(h OMECs)infected with Candida albicans(C.a),and its inhibitory effect on C.a infected oral mucosal epithelial cells.Methods: 1.To observe the effect of rh IL-17 A on the proliferation of C.a in the progress of C.a infected oral mucosal epithelial cells.The effect of rh IL-17 A on the growth and morphological structure of C.a was observed by laser confocal microscopy after further treatment with 100 ?g / L rh IL-17 A for24 hours.2.h OMECs were stimulated with different concentration(0,1,10,100,1000 ?g/L)of recombinant human IL-17A(rh IL-17A)for 48 hours,separately,and then RT-PCR and ELISA tested the expression of h BD-1,2,3.3.The h OMECs were divided into blank control group;IL-17 A alone group(rh Il-17A);C.a group(model group);C.a and rh IL-17 A co-treatment group(experimental group).The model of C.a infected oral mucosal epithelial cells was established with a yeast-to-epithelial cells ratio of 1:10 for 2 hours,and then were treated with rh IL-17A(100 ?g/L)for 24 hours,supernatant washarvested at the indicated time to determine the C.a adherence rate and the level of h BD-1,2,3 by ELISA.The expressions of h BD-1,2,3?S100A8?LL-37 m RNA were quantified by RT-PCR analysis.Results: 1.The study showed that the budding yeast could transform into hyphae,which mediate C.a infected with h OMECs.We observed a prominent effect of rh IL-17 A inhibite C.a growth and flamentated.No colonies and hyphae could be found in experimental group,which was treated with rh IL-17 A,by confocal laser scanning microscopy.2.C.a adherence rate of experimental group was lower than that of the control group,but there was no statistical differences(P>0.05).3.The results of RT-PCR showed that rh IL-17A(10,100,1000 ?g/L)could significantly induce h BD-2 expression in h OMECs,compared to blank control group,respectively(P<0.05).The results of ELISA showed that rh IL-17A(1,10,100,1000 ?g/L)could induce h BD-2 expression in h OMECs,compared to blank control group,respectively(P<0.05).rh IL-17A(100 ?g/L)had the highest contributable effect at h BD-2 m RNA level and protein level.4.The expression of h BD-2 in h OMECs was up-regulated by rh IL-17A(100 ?g/L)induced and C.a(P <0.05).The inducibility of rh IL-17 A was stronger than that of C.a group,there was a statistically significant(P < 0.05).The expression of h BD-2 was significantly higher than that of C.a group and rh IL-17 A group(P<0.05).The levels of S100A8 m RNA and LL-37 m RNA were also up-regulated by rh IL-17A(P <0.05),and the expression of S100A8 m RNA was consistent with that of LL-37 m RNA.Conclusions: Rh IL-17 A can inhibit growth and reproduction of C.a and block the yeast-hyphal transition.In addition,rh IL-17 A can induce the oral mucosa epithelial cells secreted h BD-2?S100A8?LL-37,which plays a centralrole in host protection against C.a infection.