The Preliminary Studies On The Mechanism Of Anti-rheumatoid Arthritis Extract Of Caper Fruits Based On The Network Pharmacology

Capparis spinosa L.,belonging to the plants of capparidaceae,is a traditional uygur medicine.It was used to treat the various disorders for thousands years in Xinjiang Uygur autonomous region of China,especially rheumatoid arthritis,gout,frozen shoulder and skin diseases.In previous research,it was clear that the extract of Caper fruits had the biological activity of ati-rheumatoid arthritis,and the extraction process of the bioactive fraction was ascertained.Indole,flavonoids and phenolic compounds compose the active fraction and their contents are more than 50%.Based on previous studies,we used the Network pharmacology to study the mechanism of anti-rheumatoid arthritis of bioactive fraction of caper fruits and provided scientific guide for the research of innovative drugs.The studies have been built network of compounds-target-pathway by screened for targets,genes and pathways of Ginkgetin,1H-Indole-3-carbaldehyde and 4-Hydroxybenzoic acid,isolated from bioactive fraction of caper fruits.By constructing and analyzing networks,we known that three active ingredients involve 77 targets,65 genes and 53 passages related RA.Among them,the main targets have eight,namely NFκB1,FOSB,VEGFA,RELA,PLCG1,EGFR,TP53 and RXRA.The main pathways have five,namely NF-κB signaling pathway,Wnt signaling pathway,MAPK signaling pathway,JAK / STAT signaling pathway,PI3K-Akt signaling pathway and Calcium signaling pathway.Through cell experiments,Ginkgetin can inhibite activities of the NF-κB and its IC50 is about 7.5 μM.Through animal experiments,the active fraction can reduce the expression of TNF-α,IL-1β and IFN-γ.Therefore,the experimental results verify the accuracy of the network analysis.In this study,we first studied on the mechanism of anti-rheumatoid arthritis of caper fruits extract based on the network pharmacology by the perspective of prediction and analysis.