Pristimerin Inhibits Growth And Induces Apoptosis Of Gastric Cancer MGC803 And SGC7901 Cell

AIM: To investigate the effects of pristimerin on the growth and apoptosis of gastric cancer cell line MGC803 and SGC7901, and investigate its preliminary action mechanism.METHODS: MGC803 and SGC7901 cells were cultured in vitro, CCK-8 assay,Annexin V-FITC/PI staining method, cell cycle analysis were conducted to investigate the effects of pristimerin on the growth and apoptosis of gastric cancer cell line MGC803 and SGC7901 after the cells were treated with drugs, and then the mitochondrial membrane potential and Bax and Bcl-2 protein expression level were determined by Rhodamine 123 and Western blot. Human gastric cancer MGC803 cells transplantation tumor models in nude mice were established and the effect of pristimerin on the growth of transplantation tumor were observed.RESULTS: Pristimerin could inhibit MGC803 and SGC7901 cell growth with the IC50 values of 8.5 and 12.6 ?M, respectively. Annexin-V/PI staining showed that MGC803 and SGC7901 cells were induced to death with the concentration increasing of pristimerin. In addition, the cell cycle analysis experiment revealed that pristimerin could induce G1-phase arrest. The mitochondrial membrane potential was measured after treatment with pristimerin and the results indicated that the mitochondrial membrane potential was significantly decreased. Western Blot experiments showed that the expression of Bax protein increased and Bcl-2 decreased in a concentration-dependent manner after treatment with pristimerin. Pristimerin could inhibit MGC803 cells transplantation tumor growth in nude mice.CONCLUSION: Pristimerin could inhibit the proliferation of gastric cancer cell MGC803 and SGC7901 and induce apoptosis. We think that pristimerin may inhibit proliferation and induce apoptosis of MGC803 and SGC7901 cells by inducing the loss of mitochondrial membrane potential.