| Objective: To investigate the effects of Tianshu capsule on the expression of calcitonin gene-related peptide (CGRP), adenosine 2a receptor(A2aR),adenosine A1 receptor(AlR) in the trigeminal ganglion(TG) and spinal trigeminal nucleus caudalis(TNC) of migraine model rats. Methods: Forty rats were randomly divided into four groups: blank control group (BC group), sham operation group (SO group),electrical stimulation of trigeminal ganglion group (ESTG group), Tianshu capsule group (TC group). The TG and TNC were resected to detect the expression of CGRP,A2aR and AIR by REAL-TIME PCR, Western blot.Results: (1) Electrical stimulation significantly increased the expression of CGRP,A2aR and decreased the expression of AIR in the TNC, ipsilateral TG compared with the blank group and the sham operation group (P<0.05). No significant differences were detected between the sham operation group and the blank control group. (2)Pretreatment with Tianshu capsule significantly decreased the expression of CGRP,A2aR and increased the expression of A1R in the TNC, ipsilateral TG compared with the ESTG group (P<0.05). No significant differences were detected between the blank control group and the Tianshu capsule group. (3) The correlation relationships between CGRP ,A2aR and AIR in TG and TNC of migraine model rats. It is positively correlated between CGRP and A2aR and, however there is a negative correlation between CGRP and A1R (P<0.01).Conclusions: The interactions of CGRP, A1 receptor, A2a receptor are involved in production and delivery of migraine pain information. Tianshu capsule can adjust the expression of the three migraine-related substances and can possibly prevent migraine attack. |
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