Inhibition Of DMH-DSS-induced Colorectal Cancer By Liposomal Bovine Lactoferrin In Rats

Objective: Bovine LF(bLF)is a multifunctional protein that has anti-inflammatory,anti-bacterial,anti-viral,anti-tumour and immunoregulatory effects.This study was conducted to evaluate the anti-inflammatory and anti-tumour effects of liposomal bovine lactoferrin(LBLF)in a 1,2-dimethylhydrazine(DMH)/Dextran Sulphate Sodium(DSS)-induced model of carcinogenesis in Wistar rats.Methods: Wistar rats were randomly divided into three groups(Control group(water),500mg/kg/day LBLF group and 1000 mg/kg/day LBLF group).All experimental animals were injected with DMH(20 mg/kg body weight)once per week for 8 consecutive weeks.Starting at 5 weeks of age,their drinking water was supplemented with 1% DSS for one week.Starting at6 weeks of age,the rats were divided into three groups and administered water,500 mg/kg/day LBLF or 1000 mg/kg/day LBLF.All rats were sacrificed 25 weeks after the commencement of DMH administration.The tissues were examined for the presence of aberrant crypt foci(ACF)and subjected to histopathological analysis.To investigate the inhibitory effect of LBLF in different concentrations(0,1,10 and 100?g/ml)on colorectal cancer cell proliferation,to detect the expression of TNF-?mRNA after the respective application of LBLF(0,1,10 and 100?g/ml)and LPS(100ng/ml)on RKO cells,and to discuss the inhibitory effect of LBLF on inflammation Results: Rats from the 500 mg/kg/day LBLF and1000 mg/kg/day LBLF groups harboured significantly fewer colon adenomas and adenocarcinomas than rats from the control group.Moreover,the 500 mg/kg/day LBLF and 1000 mg/kg/day LBLF groups exhibited significantly fewer aberrant crypt foci than the control group.Conclusion: Lastly,we demonstrated that LBLF inhibits cell growth and TNF-? mRNA expression.These data support the hypothesis that BLF affects colorectal carcinogenesis by suppressing cell proliferation and inflammation in rats.