| Objective: To study the effect of the carboxymethyl chitosan(CMC)in promoting the repair of mice skin defect and compare with the tissue morphology of newborn mice skin.Methods: First to establish the mice model of full-thickness skin defects.Then the mice models were divided into 4 groups: CMC group,Qingdejia group,normal saline group,and the blank group.There were 10 mice in each group.Except the mice in blank group,medicines were given to the mice in the other three groups accordingly until day 21.Thirty-six newborn mice were also included into the study.The repair rate of the mice model with skin defect and the development of the newborn mice skin were measured with the Image J technique.The development of the skin hair follicle was observed with HE staining technique.The repaired morphological structure of the hair follicles of four groups on the 21 st day and the morphological structure of the hair follicles of neonatal mice were analyzed.Results: The repair rates of the skin defect at 3 and 17 day were 33.07±9.52% and 96.75±2.11% in CMC group,24.18±6.14% and 92.22±0.62% in Qingdejia group,21.06±11.59% and 87.38±2.79% in normal saline group,and 8.66±2.27% and 85.15±1.34% in blank group respectively.Comparing the rates in CMC group with those in the other three groups respectively,significant differences were found(F=11.970,17.666,8.828,P=0.006,0.003,0.000).The development of skin thickness and the hair follicle number increase with the growth day age of newborn mice.The structure of the repaired skin of four groups on 21 day was compared with the skin tissue morphology of newborn mice,the structure of the repaired skin in CMC group,Qingdejia group,normal saline group and blank group equalized the late-stage of development,the mid-stage of development,the early-stage of development and the stage of scar respectively.Conclusion: CMC may play an important role in promoting skin regenerative repair. |
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