Establishment Of Caco-2 Cell Model Of Curcumin Self Emulsifying Drug Delivery System

In this paper,the control variable method was used to screen the CurI and CurII 2 components with good solubility,D-optimal mixture design was used to optimize the formulation of curcumin SEDDS,and to evaluate the quality of the 2 components of the drug.The optimal prescription of SEDDS is Obleique Tween-20-Obleique CC497-Transcutol P=0.49:0.17: 0.33(m:m:m).Compared with bulk drug,which increased the solubility of CurI and CurII in water 80 times and 209 times,time emulsifying was 9.1 s,the average particle size was 186 nm.CurI and CurII in medicated SEDDS in the dissolution medium dissolution is high and fast,within 45 minutes cumulative dissolution has reached more than 95%,while these three components in active pharmaceutical ingredients is almost insoluble.Active pharmaceutical ingredients and medicated SEDDS all have good oil and water distribution coefficient,log P = 2 ~ 3.On this basis,the Caco-2 cell model was established to investigate the integrity of Caco-2 cell monolayer.And CCK-8 method was used to investigate the effects of Cur SEDDS cytotoxicity on Caco-2 monolayer model and the effect of Cur SEDDS on the TEER value of Caco-2 monolayer model.