Study Of Processing With Vinegar For Leading The Drug Effect On Liver Of Schisandra Chinensis Based On Metabonomics

Schisandra chinensis is the dry ripe fruit of Fructus schisandrae plants,which has the effect of benefiting the kidney and heart.The raw one specializes in astringing lung to stop cough and promoting salivation to arrest sweating,while the vinegar processed one can enhance the effect of protecting the liver.The research was mainly to compare the lignins of raw and vinegar processed one of the change of pharmacodynamics,the metabolites about bile,urine and feces.At the same time,used the new technology and method of metabonomics to discuss the effect of protecting liver with Schisandra chinensis,and reveal the system theory of"processing with rice vinegar for leading the drug effect on liver",It will provide the basic knowledge for safe,effective and reasonable use in clinical.In accordance with the relevant provisions of the 2010 edition of Chinese Pharmacopoeia,Schisandra origin and quality of the sample was identified,the results are in line with regulations.At the same time,in the basic research subject process vinegar Schisandra chinensis.Compared the contents of lignins in the raw and vinegar processed Schisandra chinensis.HPLC method was adopted to compare the contents of Schisandrin,Schisandrol B,Schisantherrin A,Deoxyschisandrin,Schisandrin B and Schisandrin C in Schisandra chinensis.Overall,the content of the lignans is increased after vinegar processed.And the ratio of Schisandrin,Schisandrol B,Schisantherrin A,Deoxyschisandrin,Schisandrin B and Schisandrin C is changed.The results showed that the ratio and amount of lignin monomer components had corresponding changes after processed by vinegar.The effect of Schisandra chinensis and the vinegar processed one to CCl4 acute liver damage:Give Schisandra chinensis and the vinegar processed one to the mice for seven days,on the seventh day after give the medicine one hour to made the mode,then take the blood and liver after sixteen hours.Using the automatic biochemistry analyzer and pathological methods system to evaluation the effect of liver protection of Schisandra chinensis and the vinegar processed one.The results showed that both Schisandra chinensis and the vinegar processed one have the effect of liver protection,and the vinegare processed one is more obvious.The research established the method of biological samples determination,including the LC-MS analysis method of Schisandra chinensis and the vinegar processed one in rat bile,urine and feces.Using the method to study the change of prototype metabolites about Schisandra chinensis and the vinegar processed one to speculate the metabolic pathways.The results showed that Schisandra chinensis and the vinegar processed Schisandra chinensis prototype eduction body outside of the drug is less.Compared to the discharge of urine,feces and bile,the accumulation of drugs in the bile prototype is higher than the urine and feces metabolism.It speculated that Schisandra chinensis mainly etabolized by bile.And the vinegar processed one have the effect of stimulative bile etabolized.Using UPLC-TOF-MS analysis technology and pattern recognition technology as the core of the metabonomics method to study the dynamic changes of the metabolic products of Schisandra chinensis and the vinegar processed one in rat plasma.The 2D-PCA sketch of CCl4 acute liver damaged after 24 h,Fructus schisandrae and the vinegar processed one have the obvious trend to the blank group.The 3D-PLS-DA sketch showes that:The effect of Schisandra chinensis after vinegar processed is more favorable than Schisandra chinensis,it illuminates the relevancy effect of "vinegar system to the liver".Combined with the 2D-PCA sketch and 3D-PLS-DA sketch,it characterizated that using the method of metabonomics further proof that both Schisandra chinensis and the vinegar processed one have the effect of liver protection,and the vinegare processed one is more obvious.At the same time,by the endogenous metabolite identification,ten possible biomarkers and three specific biomarkers between Schisandra chinensis and the vinegar processed one were fined out.Which can guide the clinical drug use.In conclusion,after processed with vinegar,the major effective component of Schisandra chinensis changed the quantitative and ratio.The effect of CCl4 acute liver damage:both Schisandra chinensis and the vinegar processed one have the effect of liver protection,and the vinegared one is more obvious;HPLC-MS analysis method was used to determine the prototype metabolites content about bile,urine and feces.The results showed that Schisandra chinensis and the vinegar processed Schisandra chinensis prototype eduction body outside of the drug is less.Compared to the discharge of urine,feces and bile,the accumulation of drugs in the bile prototype is higher than the urine and feces metabolism.It speculated that Schisandra chinensis mainly etabolized by bile,it illuminates the relevancy effect of "liver and gall together".UPLC-TOF-MS analysis technology and pattern recognition technology as the core of the metabonomics method to study the protect liver mechanism,the results showed that the effect of the vinegar processed one is more protent than Schisandra chinensis,it illuminates the relevancy effect of "vinegar system to the liver".Ten possible biomarkers were fined out to guide clinical rationally and effectively.The chemical constituents study,pharmacodynamics study,exogenous metabolic product study and the metabolomic study were clarified the connection of material foundation change and exogenous metabolites change,the system of endogenous and exogenous metabolic products and the relevancy effect of "vinegar system to the liver".Which explain the effect of protecting liver mechanism about Schisandra chinensis after vinegar processed one.and guide the clinical drug use safely,effectively and rationally.This topic is in the establishment of plasma metabolomics on the basis of the method.Use the traditional pharmacology index and the metabolomic research to reveal the biochemical changes.And provide the new idea and vision of the mechanism research and drug effect.The results showed that the metabonomics technology have a great potential in the early diagnosis of liver disease,which can provide scientific basis for clinical diagnosis.It is very worthful for further study.