The Effect Of Enhancement Of Potassium Channel TREK-1's Activity And Ginsenoside Rg1 On SAMP8's Learning And Memorial Ability

Objectives: To study the behavior of potassium channel TREK-1 in the pathological progression of Alzheimer's disease.Also,to define the effects on learning and memorial ability of SAMP8 mice after TRKE-1 has been activated,and to clear that the mechanism of potassium channel TREK-1 to improve the learning and memorial function.Additionally,to study the role of potassium channel TREK-1 on ginsenoside Rg1's intelligent effect.Methods: 1.SAMP8 mice(Senescence accelerated mouse)were used as the animal model for the study of AD,and the same month age SAMR1 mice(senescence accelerated mouse resistant 1)were used as the control group.Enzyme linked immunosorbent assay(ELISA)was used to detect the contend of Tau protein,ACh and Glu in the brain tissue of different age SAMP8 mice.Reverse transcription polymerase chain reaction(RT-PCR)and western blotting analysis(WB)were used to detect the expression of potassium channel TREK-1 in the cerebral cortex and hippocampus of SAMP8 and SAMR1 mice.2.Four experimental groups were set.Six months age SAMR1 mice were used as a control group.The other three SAMP8 mice(six months age)groups were Alpha-Linolenic acid(0.3mg/g)group,ginsenoside Rg1(0.01mg/g)group and NS group.Morris water maze was uesd to test the learning and memorial function of mice.Nissl staining and immunofluorescence staining were uesd to observe the morpholpgical changes of Nissl bodies,cortical neurons and astrocytes in the cerebral cortex.ELISA was used to detect the content of Glu in the brain tissue,RT-PCR and WB were used to detect the expression of potassium channel TREK-1,GLT-1 and NMDAR in the cerebral cortex and hippocampus.Results: 1.With the increase of the month age of SAMP8 mice,the content of Tau protein and Glu in the brain tissue increased linearly,and ACh decreased linearly.The expression of potassium channel TREK-1 in the cerebral cortex and hippocampus increased first then decreased.2.The intervention of Alpha-Linolenic acid and ginsenoside Rg1 improved the learning and memorial ability of SAMP8 mice.The constructions of Nissl bodies,neuron and astrocyte in the cerebral cortex have been rebuilt.With the content of Glu in the brain tissue reduced,the expression of GLT-1 in the cerebral cortex and hippocampus raise.Also,the expression of NMDAR decreased.Conclusions: 1.Potassium channel TREK-1 involved into the pathological progression of Alzheimer's disease,And the function of potassium channel TREK-1 to improve learning and memorial ability was related to the regulation of glutamate metabolic pathway.2.The activated potassium channel TREK-1 improved the learning and memorial ability of SAMP8 mice that also related to ginsenoside Rg1's intelligent effect.