Detection And Significance Of Peripheral Leukocyte In Patients With Coal Workers’ Pneumoconiosis By Five-Color Flow Cytometry

Objectives Coal workers pneumoconiosis(CWP)is a kind of diffuse lung tissue fibrosis diseases.Due to long-term inhalation of coal dust and/or silicon dust.Studies have shown that dust particles into the body causes macrophage activation or damage,release the cytotoxicity oxidant,protease,cytokines,prompting inflammatory cells reach the surface of the alveolar epithelial cells and causing alveolar inflammation and injury,and inflammatory cells release toxic oxygen derivatives and proteolytic enzymes,further causing cell DNA damage,cell apoptosis and extracellular matrix deposition.We test peripheral blood cells including peripheral granulocytes,CD16+ monocytes,CD16-monocytes,B lymphocyte,cytotoxic T+NK cells,non cytotoxic T cells and so on more 16 kinds of leukocyte subgroup,further discuss the role of various cellular level changes in stage I CWP,and provide a new way of thinking for the mechanism research,clinical diagnosis and the treatment of CWP.Methods 1 To select 66 cases of stage I CWP patients from 2015-2016 years in the Institute of Occupation Disease Prevention and Cure of Kai Luan(CWP group),which were diagnosed according to "pneumoconiosis diagnosis standard"(GBZ-2009).To select47 cases of dust-exposed miner which in the same dust conditions with stage I CWP patients(dust-exposed group)and 40 cases healthy persons which were non dust-exposed in Kailuan Group(normal group).The gender,age,dust-exposed year are matched between the three groups.All cases were collected 2 ml fasting venous blood which were reserved with EDTA-K2 anticoagulant tubes in the early morning,the blood of stage I CWP hospitalized patients were collected before treatment.Five-color flow cytometry were applied to detect the level of peripheral granulocytes,CD16+ monocytes,CD16-monocytes,B lymphocyte,cytotoxic T+NK cells,non cytotoxic T cells and so on 16 kinds of leukocyte subgroup of all cases.2 To analysis date by using SPSS 17 statistical software.If follows the normal distribution,the date were described with mean±standard,then the difference between two groups was compared with One-Way ANOVA.If do not follows the normal distribution,the date were described with median and quartile range.The difference between two groups was compared with Mann-Whitney U test.The difference among three groups were compared with Kruskal-Wallis H test,the difference between two groups among three groups were compared with Nemenyi test.With P<0.05 as the difference was statistically significant.Results 1 The changes of peripheral leukocyte subsets in stage I CWP patients:Compared with normal group,the level of B lymphocyte,CD16-monocytes,total monocytes,eosinophils,basophils in CWP group were significantly higher,the difference was statistically significant(P<0.05).Compared with the patients in dust-contacted group,the level of B lymphocytes and eosinophils significantly increased,cytotoxic T+NK significantly reduced in CWP group,difference was statistically significant(P<0.05).The level of cytotoxic T+NK cells,total monocytes,CD16-monocytes,CD16+ monocytes in dust-contacted group were higher than normal group,the difference was statistically significant(P<0.05).Neutrophils in CWP is on the decline.Comparison between other indicators,there was no statistically significant difference(P>0.05).2 The changes of peripheral blood leukocyte subsets in patients with simple CWP and CWP complicated with COPD.The level of peripheral leukocyte in two groups had no statistically significant difference(P>0.05).3 The changes of peripheral blood leukocyte subsets in patients with stage I CWP in dust exposure years and in dust exposure category.The results comparison between two groups of stage I CWP patients who worked in dust-exposed condition for less than 30 years and greater than 30 years,the level of peripheral leukocyte in two groups had no statistically significant difference(P>0.05).The results comparison between two groups of CWP patients who engaged in coal dust and silicon dust,the level of peripheral leukocyte in two groups had no statistically significant difference(P>0.05).4 The changes of peripheral blood leukocyte subsets in patients with stage I CWP in different severity of lung function.The results comparison among patients with CWP who had different severity of lung function,the level of peripheral leukocyte among three groups had no statistically significant difference(P>0.05).5 The changes of peripheral blood leukocyte subsets in smoking patients with stage I CWP.The level of peripheral blood lymphocyte,B lymphocyte significantly increased,mature neutrophils significantly reduced in smoking patients than that in nonsmoking patients,difference was statistically significant(P<0.05).Comparison between other indicators,there was no statistically significant difference(P>0.05).Conclusions 1 B lymphocytes,cytotoxic T+NK cells,CD16+ monocytes,CD16-monocytes,neutrophils,eosinophils,basophils may participate in the development of CWP.2 CD16+,CD16-monocytes levels may be associated with dust stimulation,The increase of eosinophils,B lymphocyte may be associated with fibrosis of lung injury,cytotoxic T+NK cells may throughout participate in the whole process of the development of CWP.The level changes of peripheral leukocyte subsets in patients with stage I CWP may be influenced by smoking.