Changes Of Immune Function Before And After Treatment With R-CHOP And CHOP In Patients With DLBCL

Objective: Observe the changes of serum immunoglobulin,T lymphocyte subsets,beta 2microglobulin(?2-MG)and CD4 + CD25 + regulatory T cells(Treg)in peripheral blood of patients with diffuse large B cell lymphoma(DLBCL)during different chemotherapy,in order to study the influence of rituximab combined with CHOP(R-CHOP)regimen on the immune system in patients with diffuse large B cell lymphoma.Materials and Methods: 48 cases of initial treatment diffuse large B cell lymphoma accepting the R-CHOP regimen in our hospital from September 2014 to January 2017 were selected as the study group and another 44 cases accepting the CHOP regimen were selected as the control group.Observe the changes of serum immunoglobulin,T lymphocyte subsets,beta 2 microglobulin(?2-MG)and CD4 + CD25 + regulatory T cells(Treg)in peripheral blood of patients before treatment and after every chemotherapy cycle.Results:(1)Before treatment,the levels of immunoglobulin IgA,IgG and IgM in the experimental group and the control group were all lower than the normal level.The IgA,IgG and IgM levels after treatment in the experimental group were slowly decreased,the IgM level after 3 treatment courses was lower than that before treatment(P<0.05),the IgA,IgG and IgM levels after 4 treatment courses in the study group were significantly lower than those before treatment(P<0.05).The levels of IgA,IgG and IgM decreased in the control group during the therapeutic process,but the difference was not statistically significant(P > 0.05).After 5 courses of treatment,the IgA,IgG,IgM in the experimental group were significantly different from those in the control group(P < 0.05).(2)Before treatment,the levels of CD3,CD4,CD8,CD4/CD8 in the experimental group and the control group have no difference,the CD3,CD4,CD4/CD8 levels were lower than the normal level,CD8 higher than the normal level.The CD3,CD4 and CD4/CD8 levels after the implementation of R-CHOP in the experimental group were slowly decreased,while CD8 levels increased gradually.The CD3 and CD8 levels after 1 treatment course in the experimental group were significantly different from those before treatment(P<0.05),while CD4 and CD4/CD8 levels after 2 treatment course in the experimental group were significantly different from those before treatment(P<0.05).The CD3,CD4,CD8,CD4/CD8 levels in the control group did not change significantly during the treatment.CD3,CD4,CD8,CD4/CD8 in the experimental group after 2 courses of treatment compared with the control group,the difference was statistically significant(P < 0.05).(3)Before treatment,the level of ?2-MG in the experimental group and the control group were all higher than the normal level.After 1 course of treatment,the results were significantly decreased,compared with before treatment,the difference was statistically significant(P <0.05).After treatment,the level of ?2-MG decreased,the difference between the two groups have no statistical significant(P > 0.05).(4)Before treatment,the level of Treg in the experimental group and the control group were all higher than the normal level and the level of Treg in peripheral blood decreased after chemotherapy,and the difference was statistically significant(P < 0.05).Treg levels were reduced in the experimental group and the control group after treatment,the difference between the two groups was not statistically significant(P > 0.05).Conclusion:The immune function of patients with DLBCL was significantly inhibited,after R-CHOP chemotherapy,the patient's immune globulin decreased significantly,T lymphocyte subsets disorder,the body's immune function damage.