The Study Of Antitumor Effect Of Double Regulated Oncolytic Adenovirus Harboring HGFK1 Gene On Human Gastric Carcinoma Xenograft In Nude Mice

Objective To investigate the antitumor effect of double regulated oncolytic adenovirus harboring hepatocyte growth factor kringle domain 1(HGKF1)gene on human gastric carcinoma(GC)xenograft in nude mice.Methods Human GC cell SGC7901 were injected subcutaneously into nude mice to establish the human GC xenograft model.Then intratumoral therapeutic injection of double regulated oncolytic adenovirus harboring HGFK1 gene(TH-AD-HGFK1),double regulated oncolytic adenovirus(TH-AD),oncolytic adenovirus(AD5),and PBS were operated on mice respectively.Tumor growth and the survival time of mice were observed.Results Observation of the tumor volume at 28 th days after therapeutic injection showed that data of experimental(TH-AD-HGFK1)group was 1563.61±294.47mm3,TH-AD group was 2674.55±408.92mm3,AD5 group was 2792.94±533.57mm3,and PBS group was 3372.22±421.26mm3,P value of the experimental group comparing with other groups was 0.029,0.020,0.004 respectively,the experimental grouptumor volume of experimental group was statistically lower than the other groups(P<0.05).Examination of the tumor weight after executing the mice showed that data of experimental group was 3.31±0.44 g,TH-AD group was 3.59±0.34 g,AD5 group was 3.66±0.41 g,and PBS group was 3.91±0.48 g,P value of the experimental group comparing with other group was 0.239,0.140,0.013 respectively,tumor weight of experimental group was statistically lower than PBS group(P<0.05),while there was no obvious distinction between the other groups(P>0.05).All of mice in experimental group were survival for following 78 days(100%),comparing the survival rate of TH-AD and AD5 groups were both 60%,and the survival rate of PBS group was 0%.Conclusion Antitumor efficacy of Double regulated oncolytic adenovirus harboring HGFK1 on human GC xenograft in nude mice was corroborated,and it can prolong the survival time of the nude mice.