The Clinical Value Of DNA Ploidy Analysis In The Monitoring Of Cervical Intraepithelial Neoplasia

Objective: To investigate the clinical value of DNA Ploidy Analysis in the monitoring of cervical intraepithelial neoplasia.Methods: 84 patients with cervical intraepithelial neoplasia,and 30 patients with chronic cervicitis,both were chosen randomly from Beijing friendship hospital in2015 from April to December.These patients were connducted for human papilloma virus(HPV)detection,multi-point biopsy under colposcopy and DNA image cytometry.Then we examined HPV,DNA image cytometry and colposcopy biopsy at the time of 6 months and one-year after treatment.We monitored the residual and the recurrent cervical lesions to evaluate the effect of the program after treatment.Results:1.With the increasing of the degree of cervical lesions,the positive rate of HR-HPV and DNA ploidy analysis also increased.The number of DNA aneuploid cells were positively correlated with the degree of cervical lesions.2.The degree of cervical lesions,the positive rate of HR-HPV and DNA ploidy analysis were all significantly lower at the time of 6 months and one-year after the operation.3.Before the treatment,45/78 cases with the high risk type HPV infection had Positive expression in DNA image cytometry.Half a year later,7/10 CIN with the high risk type HPV persistent infection had positive expression of DNA image cytometry.A year later,4/5 CIN with the high risk type HPV persistent infection had positive expression of DNA image cytometry.4.It was 96.43% that positive DNA ploidy predicting the sensitivity of cervical5.lesions above CIN II in ASCUS patients.Conclusions:1.With the increaseing of cervical lesions,the infection rate of HR-HPV,the positive rate of DNA ploidy analysis,and the number of DNA aneuploid cells increased.2.The surgery could effectively reduce the degree of cervical lesions,the positive rate of HR-HPV and the positive rate of DNA ploidy analysis.3.DNA ploidy analysis can improve the sensitivity of diagnosis and reduce the rate of missed diagnosis in those CIN patients With HR-HPV persistent infection.DNA ploidy analysis can predict the progress of cervical lesions and monitor the prognosis of cervical lesions.4.DNA ploidy analysis has a high clinical value in the diagnosis and treatment of ASCUS with HR-HPV,which is helpful for early detection and early treatment.