The Effects Of A Disintegrin And Metallopeptidase Domain 17 On The Progression Of Brainstem Gliomas In Juvenile And Adult Mice

Objective: Our previous study in vitro has proved that A Disintegrin and Metallopeptidase Domain 17(ADAM17)promotes the ability of invasion and migration of glioma cells.The present study was designed to develop a brainstem glioma model in the juvenile and the adult mice,furthermore,to investigate and clarify the tumor growth progression and prognosis with different expression levels of ADAM17 in different microenvironments with different ages.Materials and methods:Firstly,we knocked down the ADAM17 expression of GL261-NC cell line by RNA interference(RNAi)to get a new mice glioma cell line GL261-D.Then the two group cells were stereotactically implanted into pontines of the young and the adult mice separately.Then,mice were taken magnetic resonance imaging(MRI)examination to get the information about the tumors in 8 days later after the implantation.Meanwhile,we weighted the mice and observed the changes of their behaviors everyday and recorded the time of death.Finally,we manipulated the data to get the results.Results:High expression of ADAM17 can significantly promote the growth rate of mice brainstem gliomas(p<0.01).Gl261-NC brainstem gliomas growed faster and the symptoms appeared earlier.Evenmore,the survival period is shorter.But the mice suffering tumors with low expression of ADAM17 had relatively good prognosis.The different microenvironments age had no significant effects on tumor volume(p > 0.05).Otherwise,the survival period(after implantation)had significant differences,which is closely related to the expression of ADAM17 and the age of mice(p< 0.05).Conclusion:We have successfully established the juvenile and the adult mice brainstem tumor models.The present study demonstrated that the brainstem glioma with high expression of ADAM17 progresses faster than that of low expression.