The Diagnostic Value,Expression And Prognosis Significance Of CEACAM6 In Cholangiocarcinoma

Objective: Study the diagnostic value of tumor marker CEACAM6 in cholangiocarcinoma.To investigate the expression of CEACAM6 in cholangiocarcinoma tissues and its clinical significance to prognosis.Methods: Collection 70 cases cholangiocarcinoma paraffin block specimens by pathologically confirmed after operation with complete clinical information in the Second Hospital of Hebei Medical University,2009-2014,(There were 62 cases of adenocarcinoma,mucinous adenocarcinoma in 3cases,papillary adenocarcinoma in 3 cases,squamous cell carcinoma in 1cases,neuroendocrine carcinoma in 1 cases)and collection 15 cases non tumor bile duct epithelial tissue a negative control.Collection 70 cases cholangiocarcinoma serum specimens by pathologically confirmed after operation with complete clinical information in the Second Hospital of Hebei Medical University,2014-2016,and collection 15 Serum samples from patients with cholangitis as the control group.1.Enzyme linked immunosorbent assay(ELISA)was used to detect the serum CEACAM6 levels in 70 patients with cholangiocarcinoma and in the control group of 15 cases.According to the highest point of the sensitivity and specificity of ROC curve,The cut-off values and area under curve(AUC)of CEACAM6 was obtained by receiver operating characteristic(ROC)curve.The diagnostic efficiency of the tumor markers for cholangiocarcinoma was assessed by the fourfold table.2.Using the immunohistochemistry(S-P)method to detect the expression of CEACAM6 protein in 70 cases cholangiocarcinoma tissue and 15 cases non tumor bile duct epithelial tissue.Combined with the patient’s age,tumor size,lymph node metastasis,tumor differentiation,clinical stage and other clinical factors were analyzed.Spss21 statistical software was used to analyse the results.Chi-square tests or Fisher exact test was used to analyse the rate.x ±swas used to represent data.Follow up was followed by outpatient review,telephone and letters and visits.The follow-up rate was 100%.Kaplan-Meier survival curve method for calculating the survival rate of patients with log-rank test.P <0.05 was statistically significant.Result: Enzyme linked immunosorbent assay(ELISA)showed that the serum level and positivity rate of CEACAM6 in cholangiocarcinoma patients were higher than those Cholangitis(P<0.05).Based on the ROC curve,the cut-off values and AUC of CEACAM6 were 0.1851 ng/ml,and CEACAM6,CA19-9,CEA area under the ROC curve were calculated separately as0.835,0.870,0.828.95% confidence interval ranged 0.758-0.912,0.803-0.936,0.749-0.906.In the cholangiocarcinoma patients,the diagnostic sensitivities of the tumor markers decreased in the order of CEACAM6>CA19-9>CEA(P<0.05),and the specificity in the order of CA19-9>CEACAM6>CEA(P<0.05).The expression of CEACAM6 in cholangiocarcinoma(87.1%)was significantly higher than that in non-tumor bile duct epithelium(20%)(P<0.01).CEACAM6 expression significantly correlated with clinical stage,and tumor grade.The positive rates of CEACAM6 was significantly higher in the stageⅢ+IV,lymph nodes metastasis and moderately or poorly-differentiated group than those in stageⅠ+II group,no lymph nodes metastasis and welldifferentiated group(P<0.05).The survival time of low expression of CEACAM6 group were significantly longer than those of high expression of CEACAM6 group.Conclosion:1 The high expression of CEACAM6 in cholangiocarcinoma in serum,and the positive rate was significantly higher than that of normal bile duct carcinoma group,statistical analysis showed that significant.Serological diagnosis of CEACAM6 are closely related with cholangiocarcinoma benign and malignant,which can provide ideas for early diagnosis of bile duct carcinoma.2 The results of ROC curve analysis and diagnostic value of CEACAM6 showed that Using enzyme linked immunosorbent assay(ELISA),we come to the conclusion that compared with CA19-9 and CEA,CEACAM6 has high diagnostic value and the highest sensitivity,but the specificity is low.In general,it can not only be used as a tumor marker,but also can be combined with CA19-9 and other tumor markers to detect the cholangiocarcinoma,so it can provide better reference for the early diagnosis of cholangiocarcinoma.3 CEACAM6 protein was highly expressed in cholangiocarcinoma tissues,and the positive rate was significantly higher than that in non cancerous normal bile duct tissues,statistical analysis showed significant.Therefore,the high expression level of CEACAM6 in the protein expression in cholangiocarcinoma tissues and the expression of serum concentration has statistical significance,these results suggest that CEACAM6 can be used as a tumor marker for early diagnosis of cholangiocarcinoma.4 The expression of CEACAM6 protein in cholangiocarcinoma tissues was not correlated with age,sex and tumor size,but was related to the degree of tumor progression,lymph node metastasis and tissue differentiation.It can be used to judge the malignant biological behavior of bile duct carcinoma,and its expression level is closely related to the clinical course of cholangiocarcinoma.5 The expression level of CEACAM6 protein in cholangiocarcinoma was correlated with the prognosis of patients.CEACAM6 may be an important biomarker in carcinogenesis,progression and prognosis of cholangiocarcinoma.