| Objective: Diabetic retinopathy(diabetic retinopathy,DR)is the most serious chronic microvascular complication of diabetic eye disease,one of the most important blinding disease in working age population.DR as one of the most common microvascular complication of diabetes mellitus,is type 2diabetes mellitus(diabetics mellitus,DM)reflected in the retina of the damage of metabolic disorders and endocrine system and blood system.Many factors involves in diabetic retinopathy.Individual differences,environmental factors and genetic factors affect the degree of diabetic retinopathy besides duration of diabetes,blood sugar level and blood flow theology.In recent years,with the rapid development of molecular biology,many scholars analyze the correlation between gene polymorphism,genetic regulation and diabetic retinopathy.The key role of vascular endothelial growth factor(vascular endothelial growth factor,VEGF)and its receptor(vascular endothelial growth receptor,VECFR)has been demonstrated.Studies have shown that single nucleotide polymorphism(single nucleotide polymorphism,SNP)of VEGFR-2 gene +1192C/T,-604T/C is related with the risk of many diseases.This study applies case control study,discusses the correlation between SNP of VEGFR-2 gene +1192C/T,-604T/C and the risk of diabetic retinopathy,and elucidates the molecular biological mechanism of diabetic retinopathy.Methods: This study collected 114 cases of healthy adults and 324 patients with type 2 diabetes,and according to the international WHO diagnostic criteria for diabetes and international diabetic retinopathy classi fication standard are divided into the Control group,DM group,NPDR group and PDR group four groups.Leukocyte DNA was extracted from 5ml peripheral blood of every patient.The genotype of VEGFR-2 gene +1192C/T,-604T/C were analyzed by PCR-LDR.Data were analyzed using IBM SPSS Statistics 21 Edition package,P<0.05 was considered statistically significant.Hardy-Weinberg equilibrium analysis to test the genotype frequencies of group representation.Measurement data were analyzed by variance significant.Comparison of the VEGFR-2 genotype,allelotype distribution in patients and healthy controls was performed by means of two-sided contingency tables using Chi-square test.The relative risk ratio(odds ratio,OR)and 95 %confidence interval(confidence interval,CI)were calculated by unconditional logistic regression.Results:1 The allelotype frequencies and the genotype frequencies of VEGFR-2in the Control groups were found in Hardy-weinberg equilibrium(P>0.05).2 Association between the VEGFR-2 +1192C/T SNP and diabetic retinopathy:Three genotypes of CC,CT,TT in four groups were found in VEGFR-2 +1192C/T.Genotype frequencies in Control,DM,NPDR,PDR groups are 72.8%,25.4%,1.8%;79.7%,19.5%,0.8%;73.3%,24.8%,1.9%;79.2%,19.8%,1.0%,respectively.And the results have not significant deviation(x2=2.769,P=0.837).There is no significant deviation between the four groups(P>0.05).The C and T allele frequencies in Control,DM,NPDR,PDR groups are 85.5%,14.5%;89.4%,10.6%;85.7%,14.3%;89.1%,10.9%,respectively.And the results have not significant deviation(x2=2.678,P=0.444).There is no statistical significance between the four groups(P>0.05).Compared with the CT+TT genotype,the CC genotype may not increase the onset risk of diabetic retinopathy.DM group compared with the Control group by age,gender,fasting blood glucose,systolic blood pressur of correction the odds ratio is 1.464(95%CI=0.801-2.675);between the Control group and NPDR group is 1.027(95%CI=0.565-1.867);between the Control group and PDR group is 1.419(95%CI=0.746-2.698).Compared with the C allelotype,the T allelotype may not increase the onset risk of diabetic retinopathy.The odds ratio between the Control group and DM group is 1.431(95%CI=0.827-2.479);between the Control group and NPDR group is 1.015(95 % CI=0.595-1.733);between the Control group and PDR group is 1.378(95%CI=0.768-2.472).3 Association between the VEGFR-2-604T/C SNP and diabetic retinopathy:Three genotypes of CC,CT,TT in four groups were found in VEGFR-2-604T/C.Genotype frequencies in Control,DM,NPDR,PDR groups are17.6%,45.6%,36.8%;12.2%,50.4%,37.4%;7.6%,53.3%,39.1%;7.3%,46.9%,45.8%,respectively.And the results have not significant deviation(x2=2.769,P=0.837).There is no statistical significance between the four groups(P>0.05).The C and T allele frequencies in Control,DM,NPDR,PDR groups are 40.4%,59.6%;37.4%,62.6%;34.3%,65.7%;30.7%,69.3%,respectively.And the results have not significant deviation(x2=2.678,P=0.444).There are no significant deviation between the Control group and the DM group(x2=0.434,P=0.572)and between the Control group and NPDR group(x2=1.568,P=0.238).The C allele in PDR group was significantly lower than that in group Control,and there is significant deviation(x2=4.190,P=0.041).Compared with the CT+TT genotype,the CC genotype may not increase the onset risk of diabetic retinopathy.DM group compared with the Control group by age,gender,fasting blood glucose,systolic blood pressur of correction the odds ratio is 1.212(95%CI=0.727-2.019);between the Control group and NPDR group is 1.363(95%CI=0.801-2.319);between the Control group and PDR group is 1.052(95%CI=0.610-1.814).Compared with the T allelotype,the C allelotype may reduce the onset risk of diabetic retinopathy.The odds ratio between the Control group and DM group is 0.883(95 %CI=0.610-1.278);between the Control group and NPDR group is 0.883(95%CI=0.610-1.278);between the Control group and PDR group is 0.656(95%CI=0.437-0.983).Conclusions:l In the Chinese Han population in Shijiazhuang,the VEGFR-2 gene+1192C/T,-604T/C polymorphism and the risk of DM no correlation.2 In the Chinese Han population in Shijiazhuang,the VEGFR-2 gene+1192C/T,-604T/C polymorphism and the risk of NPDR no correlation.3 In the Chinese Han population in Shijiazhuang,the VEGFR-2 gene+1192C/T polymorphism and the risk of PDR no correlation,the VEGFR-2gene-604T/C polymorphism with PDR risk,C allele can reduce the occurrence of the risk of PDR. |
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