Investigate The Effect Of Human Urinary Kallidinogenase Injection On The Expression Of Apoptosis Related Factor In Patients With Acute Cerebral Infarction

Objective: To investigate the effect of human urinary kallidinogenase(HUK)on the expression of Bcl-2 protein and Cyt C in the serum of patients suffering from acute cerebral infarction(ACI),and to explore the possible mechanism of inhibiting apoptosis of HUK.Methods: 100 patients with ACI in The Second Hospital of Hebei Medical University were collected in the study.Then they were randomly divided into HUK group and conventional group.The NIHSS was evaluated at the first day,the 14 th day and the 3rd month.Besides,we collected 5ml fasting venous blood,and the concentration of Bcl-2 protein and Cyt C in serum were tested by ELISA.Results:1 General information: 100 patients with ACI were collected in the study,all the patients met the criteria.There was no difference in the basic situation,such as age,gender and education level(P>0.05),so the two group are comparable.But 22 patients were relieved from the experiment before the14 th day because of the aggravation of the disease and the poor compliance.45 patients accepted HUK,while the other 33 were assigned as control.2 Evaluation of clinical efficacy: There was no difference in the NIHSS score of admission.But the score had dropped obviously at the 14 th day and the 3rd month.Furthermore,there was a significant difference between before and after treatment(P<0.05).And the effect of the HUK group was much larger than the conventional group(P<0.05).3 The concentration of Bcl-2 protein: There was no difference in serum Bcl-2 protein level between the two groups at first(P>0.05).As time went on,the concentration of Bcl-2 protein in HUK group first increased and thendeclined,there was a significant difference between before and after treatment(P<0.05).However,the concentration of Bcl-2 protein had been declining in conventional group,there was no significant difference between before and after treatment(P>0.05).The difference was obviously significant at the 14 th day between the two groups(P<0.05),there was a significant difference at the 3rd month(P<0.05).4 The concentration of Cyt C: There was no difference in serum Cyt C level between the two groups at first(P>0.05).As time went on,the concentration of Cyt C in HUK group first declined and then increased,there was a significant difference between before and after treatment(P<0.05).The concentration of Cyt C first increased and then declined in conventional group,there was no significant difference between before and after treatment(P>0.05).The difference was obviously significant at the14 th day between the two groups(P<0.05),whereas there was no significant difference at the 3rd month(P>0.05).Conclusions:1 HUK can significantly reduce the neurological deficit symptoms in patients with ACI,and still have a obvious effect at the 3rd months.2 HUK can inhibit the apoptosis of nerve cells and improve the nervous function.3 By regulating the Bcl-2 protein in the serum and reducing the release of Cyt C from mitochondria to cytoplasm,HUK could suppress the caspase cascade and inhibit the apoptosis of nerve cells.