Primary Research Of Hyperuricemia Rat Model

Hyperuricemia is a heterogeneous disease caused by disturbance of purine metabolic and/or reducing of uric acid excretion,the main characteristic is increasing of serum uric acid.Hyperuricemia can be divided into two kinds: primary and secondary,the majority of primary hyperuricemia.In recent years,the incidence of hyperuricemia is increasing year by year,but its etiology and pathogenesis is still unclear.A suitable animal model is the basis for the study of such diseases,however,a large number of experimental animals are mice,due to the existence of the uricolase in the rats purine catabolism pathway of purine is different from humans,the establishment of hyperuricemia animal model has brought great difficulties.At present,the commonly used modeling methods include the direct addition of uric acid precursors or exogenous uric acid,the elimination or inhibition of urate enzyme activity,inhibition of renal excretion of uric acid and so on.These methods have their own characteristics,but the maintenance time is short,the kidney injury appears early and serious,and there is still a gap between the occurrence and development of human primary hyperuricemia.Therefore,how to establish an animal model of hyperuricemia,which is consistent with the abnormal metabolism of uric acid in human body,is worthy of continuous theoretical research and experimental exploration.This experiment using fructose and potassium oxonate combined,in order to establish a stable,durable,small hyperuricemia model of liver and kidney damage in rats,to provide a suitable animal model for the study of the treatment of this diseases and drug screening.1.To investigate whether 5% fructose +OAPS can be used to establish a rat model of hyperuricemia.15 male SD rats were randomly divided into control group(n=5),10% fructose+OAPS group(n=5),5% fructose+OAPS group(n=5).The control group fed on standard diet,the 10% fructose+OAPS group fed on 10% fructose water,normal food and hypodermic injection of OAPS 100 mg/(kg·d)in the morning and evening,the 5% fructose+OAPS group fed on 5% fructose water,normal food and hypodermic injection of OAPS 100 mg/(kg·d)in the morning and evening,The rats had free access to get food and water for 13 weeks,cut the rats tail at different time points to get blood for detecting of serum uric acid(UA),blood urea nitrogen(BUN),creatinine(Cr)levels.At the end of the experiment,the rats' liver and kidneys were taken to make pathological sections.The results showed that 5% fructose +OAPS could be used to establish the animal model of hyperuricemia.2.Objective to investigate the persistence,stability and liver and kidney injury of 5% fructose +OAPS combined with hyperuricemia animal model.40 male SD rats were randomly divided into control group(n=10),5% fructose+OAPS group(n=10),5% fructose group(n=10),OAPS group(n=10),the control group fed on standard diet,the 5% fructose+OAPS group fed on 5% fructose water,normal food and hypodermic injection of OAPS 100 mg/(kg·d)in the morning and evening,the 5% fructose group fed on 5% fructose water and normal food,the OAPS group fed on standard diet and hypodermic injection of OAPS 100 mg/(kg·d)in the morning and evening,all the rats fed 18 weeks,cut the rats tail to get blood for detecting of serum uric acid(UA),blood urea nitrogen(BUN),creatinine(Cr)levels at the 1,2,4,6,8,10,12,14,16,18 week.At the end of the experiment,the rats' liver and kidneys were taken to make pathological sections.At the first week,the 5% fructose+OAPS group of serum UA levels increased rapidly,that compared with the control group,5% fructose group,OAPS group were significantly higher(P<0.01),and this phenomenon lasts for 18 weeks.The 5% fructose group and OAPS group of serum UA levels compared with the control group were significantly higher only at the second week(P<0.01).The BUN and Cr levels on the model group and control group were not statistically significant(P>0.05).The liver and kidneys of the rats were observed almost no damage.These results indicated that the combination of 5% fructose water and OAPS could be used to establish a stable,lasting,without liver and kidney damage of animal model of hyperuricemia.