BMP9 Effectively Induces Osteogenies Osteogenic Differentiation Of Immortalizied Calvarial Mesenchymal Progenitor Cell

Objective:To investigate the effect of adenovirus-bone morphgenic protein 9(Ad-BMP9)on osteogenic differentiation of the immortalized calvarial mesenchymal progenitor cells(iCALs).Methods:iCALs were infected with adenoviral vectors encoding BMP-9 or green fluorescent protein(GFP)and assessed the early osteogenic differentiation by detecting alkaline phosphatase activity after cultured for 3,5 and 7 days.After infected for 14 days,Alizarin red staining was performed to study the formation of osteogenic calcium nodules.The expression of osteogenic marker genes Runx2 and OCN was assessed by quantitative realtime(RT)-PCR and Western blot.Results:Significant increases in alkaline phosphatase activity,the expressions of Runx2 and OCN were detected in BMP-9 treated iCALs compared with GFP-treated cells(P<0.05).Meanwhile,Alizarin red staining showed that more mineralized nodules were found in the BMP-9 induced group.Conclusion:BMP-9 promotes the osteogenic differentiation of iCALs.