Effects Of DAC On The Expression Of MiR-203 And The Invasion And Apoptosis Of Cervical Cancer HELA Cell

Objective:Cervical cancer is a malignant tumor with serious threat to women health.In developing countries,cervical cancer is the second most commonly diagnosed cancer and the third leading cause of cancer death among females in less developed countries.In recent years,there are studys found that epigenetic changes in cervical cancer,especially methylation regulation,plays an important role in the occurrence and development process of the etiology of cervical cancer.Therefore,DNA methylation may control the progression of cervical intraepithelial lesions to a certain extent.Previous studies have suggested that micro RNA-203(mi R-203)is a kind of epithelial specific RNA,may played an important role in the progression from cervical precancerous lesions to cervical cancer,and its promoter in cervical cancer cell lines showed high methylation status.DAC(decitabine,5-Aza-2 deoxycytidine)as demethylation drugs,may affect the expression of mi R-203 and promoter methylation in a certain extent,thereby affecting the biology fucton of cervical cancer cells.The purpose of our research is to observe the influence of DAC on the invasion and apoptosis of cervical carcinoma HELA cell and the effect of the mi R-203 and the methylation process.Seeking for a new understanding of the mechanism and treantment of cervical cancer.Methods:Knocking down of endogenous DNMT1 by si RNA in cervical cancer cell HELA and Ha Ca T were divided into three groups:HELA cell treated with DAC as experimental group,Ha Ca T cell as negative control group and HELA cell as blank control group.Human cell lines HELA and Ha Ca T were cultured in DMEM and then treated with different concentrations of DAC(0.1UM、 0.5 UM、 2 UM、 10 UM、 50 UM)for 24 h and48h.The expression of mi R-203 was detected by reverse transcription-polymerase chain reaction,and the methylation of mi R-203 in the two kinds of cell lines was detected by methylation-specific-polymerase chain reaction(MSP).Transwell method was used to detect the invasion ability of cells,and the apoptosis was detected by flow cytometry assays.Results:The experimental group,the expression level of mi R-203 methylation level and invasion ability were lower than the blank control group,higher than the negative control group,the apoptosis rate is lower than the negative control group,higher than the control group;but with the increase of the concentration of drug increased gradually,the expression level of mi R-203 methylation level rise,decrease the ability of invasion,apoptosis rate increased significantly.Conclusion:DAC may induce the promoter methylation of mi R-203 promoter in cervical cancer HELA cells,which can increase the expression level and inhibit the cell invasion and induce apoptosis.