Application Of Oral Squamous Cell Carcinoma Using Doxorubicin-loading Mesoporous Magnetic Silia Nanoparticles In Vitro

Objective:To investigate the effect of Doxorubicin-loading Mesoporous Magnetic Silia Nanoparticles on oral squamous carcinomas cells in vitro.Methods: Oral squamous carcinomas cells were treated with various concentrations of blank Mesoporous Magnetic Silia Nanoparticles up to a maximum concentration of 100 ug/ml to evaluate its biocompatibility.Oral squamous carcinomas cells were cultured with different concentrations of Doxorubicin-loading Mesoporous Magnetic Silia Nanoparticles(0.5,1.0,5.0,and 10.0 ug/ml),Inverted microscope was used to observe the oral squamous cancer cells apoptosis morphological changes,MTT assay was used to detect the targeted cytotoxicity of blank Mesoporous Magnetic Silia Nanoparticles and DOX@M-MSNs against oral squamous cell carcinomas.With the concentrations of 10.0 ug/ml,cell cyclical was ascertained by cell flow cytometry after 24 and 48 hours.With the concentrations of 10.0 ug/ml,DOX aggregation and cell destruction were observed under the condition of in magnetic field and non-magnetic field by a confocal laser scanning microscope after 30 min,60min,90 min,120min.Result: The blank Mesoporous Magnetic Silia Nanoparticles showed excellent biocompatibility with cell viability >80% at the maximum tested concentration.With increase of concentration and the going of time,The DOX@M-MSNs on tumor cell proliferation inhibition was superior to that of free DOX and showed significant difference(p<0.05).Flow cytometry showed that DOX@M-MSNs induce higher necrotic rates than free DOX,and with the going of time,the necrosis rate increased.Laser scanning confocal microscope showed that the magnetic area of DOX @ M-MSNs in gathered is better than that of non-magnetic regions of the tumor cells through external magnetic field,with increase of time,compared with non magnetic area,the magnetic area showed a higher cell death.Conclusions: DOX@M-MSNs can selectivity recognize oral squamous carcinomas cells,enhance to deliver DOX into the cells and promote its effect in killing oral squamous carcinomas cells.By applying the magnetic field,the drug delivery efficiency of DOX@M-MSNs could also be increased.