Preliminary Study On The Effect Of Low Dose Chromium On The Proliferation Of Bladder Cancer Cells By Up-regulating FLNA

Objective To investigate the effect of low dose heavy metal chromium exposure on the progression of bladder cancer,and to explore the effect of FLNA on the expression of the gene and the related biological behavior.Methods Low doses of chromium exposure can promote the proliferation of T24 cells,and found that the chromium exposure dose was 0.4u M,the proliferation of the most significant effect(P<0.05);8u M antimony exposed T24 cells,cell migration and invasion were significantly increased(P<0.05);and immunohistochemistry showed that the expression of FLNA in invasive urinary tract epithelial carcinoma was strongly positive,and non invasive urothelial carcinoma tissue was weakly positive,and immunohistochemical results were negative,the results were statistically significant.QPCR detection of bladder cancer and adjacent tissue FLNA m RNA expression showed that muscle invasive urothelial carcinoma was higher than that of non muscle invasive urothelial carcinoma,expression of Western blot and the lowest paracancerous tissues showed normal human umbilical vein endothelial cell(HUVC)was lower than that of q PCR results in the T24 cells indicates that T24 cells the expression of FLNA in protein expression of FLNA was higher than that of HUVC Cell scratch test and Transwell.Experimental results show that the migration and invasion of FLNA knockdown cells was lower than that of untreated T24 cells.At low doses of chromium revealed Western after blot results showed that the expression of FLNA.Immunofluorescence results also showed that FLNA in low doses of chromium after exposure in the cytoplasm than without the high expression of chromium stimulated cells showed.Results Low doses of chromium exposure can promote the proliferation of T24 cells,and found that the chromium exposure dose was 0.4u M,the proliferation of the mostsignificant effect(P<0.05);low dose chromium exposure in T24 cells,cell migration and invasion were significantly increased(P<0.05);and immunohistochemistry showed that the expression of FLNA in invasive urinary tract epithelial carcinoma was strongly positive,and non invasive urothelial carcinoma tissue was weakly positive in normal tissue,immunohistochemical results were negative,the results were statistically significant.QPCR detection of bladder cancer and adjacent tissue FLNA m RNA expression showed that muscle invasive urothelial carcinoma was higher than that of non muscle invasive urothelial carcinoma,expression of.Western blot and the lowest paracancerous tissues showed normal human umbilical vein endothelial cell(HUVC)was lower than that of q PCR results in the T24 cells indicates that T24 cells the expression of FLNA in protein expression of FLNA was higher than that of H The UVC cell line.The scratch test and Transwell test results show that the migration and invasion of FLNA knockdown cells was lower than that of untreated T24 cells.At low doses of chromium revealed Western after blot results showed that the expression of FLNA.Immunofluorescence results also showed that FLNA in low doses of chromium after exposure in the cytoplasm is not after stimulation of the cells showed high expression of chromium.Conclusion Low dose chromium exposure can significantly affect the biological behavior of bladder cells and promote the progression of bladder cancer.The mechanism of this effect is the role of low dose chromium in regulating the expression of FLNA and related signaling pathways...