The Expression Of P53、Survivin And HDGF In Gastric Cancer Tissue And The Relationship Between Clinical Studies And Biological Behavior

Objective: This study was to detect P53、Survivin and HDGF in GC tissue and express in the cancer tissue adjacent to carcinoma,the expression of P53、Survivin and HDGF and the correlation between clinical pathological features and prognosis of GC and analysis the correlation of both expressed in GC.Methods: The present study included in 60 GC patients who accepted standerd D2 radical gastectomy in Hebei university affiliated hospital from 2012 January 1st to 2014 January 1st.Through collection,statistics and analysis of patients name,gender,age and pathological pattern with GC,According to the age of the patients were divided into age(60 or higher)and age(< 60 years old)two groups;According to the size of the tumor was divided ≧into 5 cm and < 5 cm in the two groups;According to the histological grading into better differentiation groups(differentiation of high + moderatel group)and low differentiation group(low + undifferentiated group).According to the tumor infiltration depth into abuse and serosa layer and outside groups,not abuse and serous layer.According to the presence of lymph node metastasis were divided into group with lymph node metastasis,without lymph node metastasis group.According to the TNM stage were divided into Ⅰ/ Ⅰand Ⅲ/ Ⅲperiod in the two groups.P53、Survivin and HDGF were analyzed using SP immunohistochemical staining,and analize the clinical biological behavior of GC about both relations.The data were analyzed using SPSS21.0,P<0.05 was recognized as statistically significance.Results: Expression and correlation analysis of P53、Survivin and HDGF protein in GC and adjacent noncancer oustissues.The positive expression rate of P53、Survivin and HDGF are 60%,63.3% and 68.33% in gastric cancer tissue,but the positive expression rate in the tissue adjacent to carcinoma are 25%,31.7% and 28.3%.Positive expression rate of P53、Survivin and HDGF in GC tissue is significantly higher than adjacent noncancer oustissues.(The P is 0.0001,0.0005 and 0.0002 respectively,X2 is 15.0384,12.0685 and 14.5814 respectively).The expression of P53 protein and the relationship between the clinical gastric cancer biological behavior in gastric cancer tissues.1)The expression of P53 protein is not associated with age(P = 0.4603)and the sex(P= 0.7479)of the patients,in GC.2)The expression of P53 protein is not associated with tumor size(P=1.0000),of the patients,in GC.3)The expression of P53 protein is associated with tumor differentiation degree(P= 0.0441),infiltration depth(P=0.0319),lymph node metastasis(P=0.0253),and TNM staging(P=0.0289)of the patients,in GC.The expression of Survivin protein and the relationship between the clinical gastric cancer biological behavior in gastric cancer tissues.1)The expression of Survivin protein is not associated with age(P=0.4071)and the sex(P=0.9704)of the patients,in GC.2)The expression of Survivin protein is not associated with tumor size(P=0.6646),of the patients,in GC.3)The expression of Survivin protein is associated with tumor differentiation degree(P=0.0192;P = 0.0202),infiltration depth,lymph node metastasis(P = 0.0251)and TNM stage(P = 0.0251)of the patients,in GC.The expression of HDGF protein and the relationship between the clinical gastric cancer biological behavior in gastric cancer tissues.1)The expression of HDGF protein is not associated with age(P=0.3568)and the sex(P=0.0.8653)of the patients,in GC.2)The expression of HDGF protein is not associated with tumor size(P=0.6119),of the patients,in GC.3)The expression of HDGF protein is associated with tumor differentiation degree(P=0.0111;P = 0.0098),infiltration depth,lymph node metastasis(P = 0.0175)and TNM stage(P = 0.0198)of the patients in GC.The correlative analysis of expression about P53 and Survivin in gastric cancer tissue.The mumber which Both P53 and Survivin are positively expressed at the same time is 32 cases in 60 cases of gastric cancer tissues,which they are negatively expressed at the same time is 18 cases;the number which have the same result is 50 cases,and 10 cases which have not the same result.the expression of P53 and Survivin in gastric cancer is consistent,and there are positively correlated(r = 0.6495,P < 0.6495).The correlative analysis of expression about P53 and HDGF in gastric cancer tissue.The mumber which Both P53 and HDGF are positively expressed at the same time is 30 cases in 60 cases of gastric cancer tissues,which they are negatively expressed at the same time is 19 cases;the number which have the same result is 49 cases,and 11 cases which have not the same result.the expression of P53 and Survivin in gastric cancer is consistent,and there are positively correlated(r = 0.6281,P =0.0008).Conclusions:1 The positive expression rate of P53、Survivin and HDGF are 60%,63.3% and 68.33% in gastric cancer tissue,but the positive expression rate in the tissue adjacent to carcinoma are 25%,31.7% and 28.3%.Positive expression rate of P53、Survivin and HDGF in GC tissue is significantly higher than adjacent noncancer oustissues.They are expected to become the diagnosis of gastric cancer molecular biological indicator.2 The positive expression rate of P53、Survivin and HDGF in GC tissue is not associated with gender,age and tumor size.3 The positive expression rate of P53、Survivin and HDGF in gastric cancer tissue is associated with the degree of tumor differentiation,lymph node metastases,infiltrating depth,and TNM staging.The higher the degree of tumor differentiation,the lower the positive rate of the above three indicators;the deeper the degree of tumor infiltration,the higher the positive rate of the above three indicators;the presence of metastatic lymph nodes in patients with gastric cancer,the positive rate of the above three indicators;TNM stage later,The higher the positive rate。4 The expression of P53 with Survivin and HDGF in GC tissue is positive correlation,all of P53,Survivin and and HDGF have the high consistency.5 P53,Survivin and HDGF play an important role in the occurrence and development of gastric cancer,in the process of invasion and trasfer,which are expected to provide early diagnosis and treatment of gastric cancer molecular biology.