Experimental Study Of Treatment Of Sarcopenia With Human Umbilical Cord Wharton’s Jelly Mesenchymal Stromal Cells

Objective:To transplant the Human Umbilical Cord Wharton’s Jelly Mesenchymal Stromal Cells(h UCMSCs)to the model mice(the model animal of Sarcopenia).Investigate the method and feasibility of cell transplantation for Sarcopenia.Methods:Animal model of Sarcopenia was built by hindlimb suspension technique.24 months-aged male C57 BL / 6 mice were randomly divided into h UCMSCs tail vein transplant、h UCMSCs muscular transplant、HBSS(Hank’s Balance Salt Saline)tail vein transplant and HBSS muscular transplant groups.USE the methods of enzymes digestion and adherent growth to obtain sufficient h UCMSCs in vitro,while inject the3 rd generation cells into model mice via the tail vein injection or gastrocnemius intramuscular injection.By comparing the maximal equiaquent plantar flexion strength、muscle wet weight,、the cross-sectional area of the muscle fibers and the ratio of type I fibers of the gastrocnemius muscle to evaluate the curative effect of h UCMSCs transplantation.Results:1.With the methods of enzymes digestion and adherent growth,we can get stable and enough cells to satisfy the need of experiment.2.Hindlimb suspension lead to weight loss in the elderly mice.And the decline continued after walk again and eight weeks h UCMSCs transplatation.3.Hindlimb suspension lead to dramatic decline of the maximal equiaquent plantar flexion strength in the elderly mice.Transplantation of h UCMSCs can significantly improve this strength,and gastrocnemius intramuscular injection is prior to the tail vein injection.4.Transplantation of h UCMSCs can significantly increase the muscle wet weight,the cross-sectional area of the muscle fibers and the ratio of type I fibers of the gastrocnemius muscle,gastrocnemius intramuscular injection is prior to the tail vein injection.Conclusion:1.hUCMSCs which are easy to culture in vitro can be transplanted into the host e by injection.2.Transplantation of h UCMSCs can significantly increase the muscle wet weight and improve hind limb muscle strength,which indicates cell transplantation can play a role in Sarcopenia animal model.