Differential Gene Expression Analysis And Gene Network Analysis In Mouse Middle And Inner Ear During Acute Otitis Media

Background Otitis media(OM)is one of the most common infectious diseases in childhood,and a variety of genes are involved in the pathogenesis.At present,there have had several studies on gene expression profiling in mouse middle and inner ear during acute otitis media,but they are all base on Haemophilus influenzae(Hi),while the studies about Streptococcus pneumonia(S.Pn)has not yet been carried out.And the study of otitis media is mainly about the traditional single gene,which is obviously can not meet the needs of the current systems biology research.Therefore,this study mainly established the gene network of otitis media caused by nontypeable Haemophilus influenzae(NTHi),screened the main network center node,and studied the genetic changes involved in mouse acute otitis media caused by Streptococcus pneumoniae.Methods The original gene microarray data of acute otitis media caused by NTHi were analyzed,and the differential gene network was constructed by bioinformatics software.The mouse model of acute otitis media cause by S.pn was established by tympanic puncture,and the mRNA expression profile was conducted for screening differentially expressed genes in mouse middle and inner ear.Results The gene expression chip(GSE66141)of mouse acute otitis media caused by NTHi was analyzed by using STRING online tools and Cytoscape software,and the gene network was then established.The microarray data of otitis media caused by S.pn revealed that 702 genes were up-regulated and 250 genes 702 genes were significantly up-regulated and 250 genes were significantly down-regulated in mouse middle ear at 24 h.140 genes were significantly up-regulated and 171 genes were significantly down-regulated in mouse inner ear at 24 h.Conclusions The results of this study may provide us a better understanding of the molecular mechanism involved in acute otitis media.