MiRNA-133a Down-regulates The Expression Of Gdnf Gene To Inhibit The Development Of Nreteric Bud

The present study predicted and testified that miR133 a could regulate Gdnf(glial cell line derived neurotrophic factor),a protein generated by metanephrogenic mesenchyme(MM),which is of great importance in the development of ureteric bud(UB)and investigated inhibitory effect of miR133 a towards the development of UB.Bioinformatics analysis displayed the high conservation of the Gdnf mRNA 3?Untranslated Regions,(3?UTR)between different species and predicted that miR133 a could probably bind to it.The results of luciferase assay confirmed that miR133 a could directly target Gdnf mRNA 3?UTR.The results of Real-time PCR and Western Blot revealed that miR133 a down regulated the expression of Gdnf both at mRNA and protein levels.Furthermore,we found significant reduction in the ability of miR133 a treated cells to contribute to ureteric bud formation in embryonic kidney rudiments culture system.Taken together,our results suggest that miR133 a might inhibit the formation of ureteric bud through its binding to complementary sequences in the Gdnf mRNA 3?UTR.