Effects Of Aminoguanidine On The Differentiation Of Osteoclasts Induced From Bone Marrow Cells Of Rats In Vitro

Objective:To explore the function of the different concentrations of aminoguanidine on osteoclast differentiation induced from bone marrow cells of rats in vitro.Methods:Culture osteoclasts induced from bone marrow cells in vitro,and add different concentrations of aminoguanidine(AG)in the first day.Tartrate-resistant acid phosphatase(TRAP)staining was performed in the tenth day of culturing,in order to observe the variety of TRAP-positive cells;Observe the resorption of bone on bone slices by scanning electron microscope in the tenth day of culturing;The expressions of mRNA in osteoclast special marks about receptor activator of nuclear(RANK)and CathepsinK(CK)were determined by RT-PCR in the tenth day of culturing;The expressions of protein about advanced glycation end products(AGEs)?Receptor for advanced glycation end products(AGER)?P38 and P65 were determined by Western-Blot in the tenth day of culturing.Results:AG can play a role in promoting osteoclast formation and the number of TRAP-positive cells increased gradually following the increased AG concentrations.Bone absorption experiment showed that AG can also promote bone lacuna formation.The results of RT-PCR demonstrated that the expressions of m RNA in osteoclast special marks about RANK and CK increased gradually with the increase ofAG concentrations.While the results of Western-Blot showed that the expressions of AGEs and AGER decreased,and the expressions of P38 and P65 increased.All these findings proved that AG can induce osteoclast differentiation and formation.Conclusion:1.AG can reduce the content of AGEs and AGER on differentiation of osteoclasts induced from bone marrow cells.In this experiment,AG can inhibit AGEs and its receptor AGER as a dose-dependent manner.2.AG can promote the formation of osteoclasts induced from bone marrow cells in vitro.Within a certain concentration range,it promoted osteoclast differentiation in a dose-dependent manner.