The Expression Level Of LIMK1 And Cofilin In Prostate Cancer Cells And The Correlations Of Them To Metastasis,Invasion,and Prognosis Of Prostate Cancer

Objective:To explore the expression levels of LIMK1 and Cofilin in prostate tissue,benign prostatic hyperplasia tissues(BPH),metastatic lymph nodes and investigate the correlations of them to patients' clinical condition,postoperative Gleason score,TNM stage,lymph node metastasis and biochemical recurrence of prostate cancer,and to probe the guiding value of them on prostate cancer lymph node dissection.Methods:The data from 279 diagnosed prostate cancer patients with radical prostatectomy from January 2012 to September 2015 were collected retrospectively,including patients' clinical condition,postoperative Gleason score,TNM stage,lymph node metastasis and biochemical recurrence of prostate cancer,immunohistochemistry was used to confirm the expressions of LIMK1 and Cofilin in prostate tissue,prostatic hyperplasia tissue and metastatic lymph nodes.The data was statistically analyzed concerning the expression levels of LIMK1 and Cofilin in prostate cancer cells and the correlations of them to metastasis,invasion,and prognosis of prostate cancer,and to probe the guiding value of them on prostate cancer lymph node dissection.Results: LIMK1were expressed in 215 of 279 PC case(77.1%),and 13 in 50 BPH case(26.0%).The LIMK1 expression was mainly located in positive cytoplasm,The positive rates of LIMK1 expression in prostate cancer tissues was significantly higher than that in benign prostatic hyperplasia tissues(P<0.001).Cofilin were expressed in 200 of 279 PC case(71.6%),and 18 in 50 BPH case(36.0%).The Cofilin expression was mainly located in positive cytoplasm,The positive rates of LIMK1 expression in prostate cancer tissues was remarkably higher than that in benign prostatic hyperplasia tissues(P<0.001).Metastatic lymph nodes of Prostate cancer expressed high levels of LIMK1 and Cofilin,with outstanding higher staining intensity index comparing with prostate cancer.The expression levels of LIMK1 and Cofilin positively correlated with prostate volume,serum PSA,PSAD,Gleason score,tumor stage,Lymph node metastasis,capsular invasion,seminal invasion(P<0.05),and showed no outstanding relation with patients' age(P>0.05).Pelvic Lymph Node Metastasis of prostate cancer positively correlated with serum PSA,Gleason score after radical prostatectomy,capsular invasion,seminal invasion,positive margins and the expression levels of LIMK1 and cofilin(P<0.05),and showed no significant relation with patients' age,BMI,prostate volume,percentage of positive biopsy cores and PSAD.(P>0.05).Logistic regression analyses showed that the expression levels of LIMK1 and cofilin were independent risk factor of the metastasis of prostate cancer.(P<0.001)The cox univariate analysis showed that high percentage of positive biopsy cores,Gleason score,capsular invasion,positive margins,the expression levels of LIMK1 and cofilin were associated with biochemical recurrence(P<0.05).Cox Multiple factors analysis show that high expression levels of LIMK1 and Cofilin indicated high biochemical recurrence of prostate cancer(P<0.05).The biochemical—free—survival curve drawn by Kaplan—Meier method showed that higher expression levels of LIMK1 was associated with shorter relapse free survival(P<0.001),and higher expression levels of Cofilin was associated with shorter relapse free survival(P<0.001)Conclusion: The expression of LIMK1 and Cofilin is correlated with pathological characteristics of prostate cancer,may predict metastasis,invasion,and prognosis of prostate cancer.If further research is carried,the expression of LIMK1 and Cofilin is expected to guiding the lymph node dissection of prostate cancer for its critical role in metastasis,invasion,and prognosis.