Effect Of Calpastatin Peptide?Calpain Inhibitor ??Calpeptin On Proliferation And Apoptosis Of C2C12 Myoblasts In Tumor Microenviroment

Objective:To investigate the effect of Calpastatin Peptide,Calpain Inhibitor ? and calpeptin on the proliferation and apoptosis of C2C12 in tumor microenvironment,and try to explore its possible mechanism.Methods:CT26 and C2C12 cells were co-cultured by transwell.The co-culture was divided into five groups: group A(Calpastatin Peptide group),group B(Calpain Inhibitor group ?),group C(Calpeptin),group D(negative control group)(Blank control group).The proliferation of C2C12 cells was detected by CCK8 method.The expression of ERK,NF-KB and P38 in C2C12 cells were detected by immunohistochemistry.The expression of ERK,NF-KB and P38 in C2C12 cells were detected by real-time fluorescence quantitative PCR.The expression of ERK,NF-KB and P38 mRNA in the cells.Results:1.The results of CCK8 showed that the absorbance of group A,B and C was significantly higher than that of group D(P <0.05).At 12 h,the absorbance of group A was higher than that of group E,(P <0.05).2.Immunohistochemistry revealed that the expression of ERK protein was significantly higher than that of group D(P <0.05).The expression of ERK protein was significantly higher than that of group D(P <0.05)(P <0.05).At 6 hours,the protein expression of group A was not significantly different from that of E group(P> 0.05).The expression of P38 protein(P> 0.05)was significantly higher than that of E group(P> 0.05),and the difference was statistically significant(P <0.05)(P <0.05).Compared with group D,the expression of histone was lower at 6-24 h(P <0.05).3.q-PCR results showed that the expression of ERK,NF-KB and P38 mRNA was significantly higher than that of D group(P <0.05).The relative expression of ERK,NF-KB and P38 mRNA was significantly higher than that of D group(P <0.05).Conclusion: Calpain inhibitor can promote the proliferation of C2C12 myoblasts in tumor microenviroment,and can inhibit the early apoptosis of C2C12 cells to a certain extent,but can not reverse its late apoptosis.Different calpain inhibitors act at different times.