Clinical Significance Of Hepatitis B Virus Large Surface Protein And Its Correlation With Virus Replication And Liver Unction Indexes

Objective To investigate the comparative study of serum hepatitis B virus(HBV)large protein(HBV-LP),HBV-DNA,and Pre S1 antigen(Pre S1-Ag)detection in the evaluation of serum HBV replication in patients with chronic hepatitis B.and Correlation between the expression level of HBV-LP and the indexes of liver function.Methods A total of 482 patients infected with chronic hepatitis B virus(CHB)were enrolled and the serums were collected in a hospital of Hefei city in Anhui province from June 2013 to March 2015.The serum HBV-LP,HBV markers(HBV-M)and Pre S1-Ag were detected using ELISA,and HBV-DNA were quantified using quantitative real-time PCR.The positive detection rate difference of HBV-DNA,HBV-LP and Pre S1-Ag were compared,the correlation between the logarithm of HBV-DNA copies number and the absorbance value of HBV-LP was analyzed using Spearman rank correlation.liver function index,alanine aminotransferase(ALT)and aspartate aminotransferase(AST)and gamma glutamyltransferase(GGT)and total bilirubin(TBIL)and direct bilirubin(DBIL)are detected by Hitachi 7600 automatic biochemical analyzer.Results(1)The positive rates of HBV DNA,HBV-LP,and Pre S1-Ag were 67.22%(324/482),73.86%(356/482),and 37.34%(180/482),respectively(P<0.01).(2)The positive rates of the three markers were 54.57%(185/339),64.90%(220/339),and27.73%(94/339),respectively,in 339 HBe Ag-negative CHB patients(P<0.01).In HBe Ag negative patients,the positive rate of HBV-LP in 185 cases of HBV-DNA positive samples was 90.81%(168/185),which was higher than that of Pre S1-Ag with rate of 39.46%(73/185)(P<0.01).The positive rate of HBV-LP was 33.77%(52/154)in154 cases of patients with negative HBV-DNA whose positive rate was higher than Pre S1-Ag with positive rate of 13.64%(21/154)(P<0.01).(3)The positive rates of HBV-DNA,HBV-LP and Pre S1-Ag in HBs Ag,HBe Ag and HBc Ab positive groups were 97.04%(131/135),94.81%(128/135),and 60.00%(81/135),(P<0.01);The positive rates of three indexes of HBs Ag,HBe Ab,HBc Ab positive group were 53.74%(122/227),63.88%(145/227),and 27.31%(62/227);The positive rates of three indexes of HBs Ag and HBc Ab positive group were 55.79%(53/95),67.37%(64/95),and28.42%(27/95)(P<0.01).(4)The absorbance value of HBV-LP was positively related with the logarithm of HBV-DNA copies number(Spearman rank correlation coefficient was 0.908,P<0.01).(5)Moreover,ALT,AST and GGT in CHB patients with positive HBV-LP were higher than that of HBV-LP negative patients,the difference was statistically significant(P<0.01).There was no significant difference between TBIL and DBIL test(P>0.05).However,the expression level of HBV-LP was not significantly different from that of ALT,AST,GGT,TBIL and CBIL(P>0.05).Conclusion There was a good correlation between HBV-LP and HBV-DNA load value,and could be used as an effective complement for the detection of HBV-DNA and HBV-M.Compared with Pre S1-Ag;The liver tissue damage in HBV-LP positive CHB group was more serious than that in HBV-LP negative group,but there was a limit to the degree of liver function impairment in patients with HBV-LP alone.