Protective Strategy And Preclinical Study Of Donor Liver Regeneration After Transplantation

Part ?Hypothermic Machine Perfusion promotes the regeneration of isolated liver.ObjectiveTo study the different preservation methods on the isolated liver injury and regeneration.Method12 Sprague-Dawley rats were randomly assigned to two groups:static cold storage(SCS)and Hypothermic machine perfusion(HMP)(n=6).Histidine-tryptophan-ketoglutarate solution(HTK)was used as preservation solution.The graft was preserved in HTK(4?)for 6 hours in SCS group,while perfused using HTK in HMP group.Liver tissue was obtained and fixed in 10%neutral formalin for immunohistochemistry.The fresh liver tissue was collected and stored in-80? for RT-PCR and WB analyses.ResultCompared to SCS,HMP improved liver regeneration ability in terms of PCNA and ki67 detection;promoted cell proliferation in PCR levels(Cdc25A,cdkl,cdk6)and WB levels(Cyclin D1,Cyclin El).ConclusionHMP is superior to SCS in liver regeneration,expected to be the ideal method for preservation of donor liver.Pwo ?The effect and significance of remote ischemic response onliver regeneration in orthotopic liver transplantationObjective:Liver transplantation has become the main method for end-stage liver disease.Our previous test established the remote effect of liver transplantation(RIC)model and confirm the donor liver after liver transplantation on the morphological structure and function has a significant protective effect.We will investigate the effect of RIC on liver regeneration after liver transplantation.Materials and methods:The SD rats were divided into sham operation group(N),orthotopic liver transplantation(OLT)and remote ischemic group(RIC).While RIC group underwent remote ischemic operation on the thigh during the anhepatic phase of recipient liver.After the portal vein was reflow for 3h before rats were sacrificed.Some liver tissues were fixed in 10%formalin for immunohistochemical detection,and the rest of the fresh liver tissues were frozen immediately at-80? for PCR and WB.Result:With the OLT group and N group,RIC group's immunohistochemical analysis of PCNA and Ki67 expression,cell proliferation assay method of RT-PCR related gene CDC25A,CDK2,Cdk6 reduced.Western blot detection of Cyclin D1 and Cyclin E1 in the liver decreased.The result of RIC group were closer to N group.Conclusion:RIC reduced ischemia-reperfusion injury and significantly improved liver function after transplantation.while,IRI reduces the regenerative capacity of the doner liver.