The Protective Effect Of Tiopronin On Acute Liver Injury In Mice Induced By Con A And Its Mechanism

Objective To investigate the protective effect of tiopronin(MPG)on acute liver injury in mice induced by concanavalin A(Con A)and its mechanism.Methods Mice were divided into 4 groups using a random number table :normal saline matched group(group NC),liver injury induced by Con A group(group Con A),low-dose tiopronin pretreatment group(group LD)and high-dose tiopronin pretreatment group(group HD).Mice of group LD and group HD were given deferent dose of MPG through intraperitoneal injection for 7 days,while mice of group NC and group Con A were given normal saline through intraperitoneal injection for 7days.Then all of the 4 groups were injected with ConA(20 mg/kg)to induce acute autoimmune hepatitis.The mice were given Con A through caudal vein to build the model of acute autoimmune liver injury,while the mice of normal saline matched group were given normal saline the same way.30 min later,deferential dose of tiopronin was given through caudal vein.Mice of all groups were killed at 2h,8h and 24 h to collect the serum samples and hepatic tissue.The pro-inflammatory cytokine and liver function levels,apoptosis and autophagy activity,the expression of ERK and Bcl-2 activity were determined 2,4,and 8 h after the ConA injection by the way of western blot?qRT-PCR and immunohistochemistry.Results The determination results of the pro-inflammatory cytokine and liver function levels,apoptosis and autophagy activity,the expression of ERK and Bcl-2 activity between the 4 groups had statistical significance in varying degrees.Conclusion The MPG has protective effeft on acute liver injury in mice induced by Con A,and it can significantly improved the apoptosis and autophagy induced by Con A.T...