| Objective: Cervical cancer is currently one of the major threats to women's health.The overexpression of heterogeneous nuclear ribonucleoprotein A2/B1(hnRNP A2/B1)as the biomarker has been investigated in various cancers.In our previous study,we found that lobaplatin induced apoptosis and cell cycle arrest via downregulation of proteins including hnRNP A2/B1 in cervical cancer cells.However,the underlying relationship between hnRNP A2/B1 and cervical cancer remained largely unknown.Methods: The expression of PI3 K,AKT,p-AKT,p21,p27,caspase-3,cleaved caspase-3 were revealed by western blot.Nude mouse xenograft model was undertaken with HeLa cells and the xenograft tumor tissue samples were analyzed for the expression of PCNA and Ki-67 by immunohistochemistry and the cell morphology was evaluated by hematoxylin and eosin(H&E).Results: The expression of p21,p27 and cleaved caspase-3 in shRNA group were significantly upregulated and the expression of p-AKT was reduced both in vitro and in vivo(P<0.05).The results of immunohistochemistry showed that PCNA and Ki-67 were significantly downregulated in vivo(P<0.05).The growth of nude mouse xenograft tumor was significantly reduced by hnRNP A2/B1 knockdown.Conclusion: These data indicate that inhibition of hnRNP A2/B1 in cervical cancer cells can inhibit cell proliferation and invasion via PI3K/AKT signaling pathway.In addition,after silencing hnRNP A2/B1 can inhibit tumor growth. |
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