E-jet 3D Printing Of Medical Scaffolds For Personalized Anti-cancer Theraphy

Cancer is among the most common causes of morbidity and mortality worldwide,the need for the development of more effective cancer diagnostics and therapeutics is imperative.In fact,the effect of pursue the massive investment in cancer therapy and in particular drug development is not ideal.Researchers are working on developing effective cancer treatments,including interventional therapy,targeted therapy and immune therapy.In the field of interventional therapy,carrier-based drug delivery systems have attracted more and more attentions.They have the ability to preferentially carry drugs into tumor tissues or to targeted locations,which is in contrast to systematic administration of free drugs.In this study,we applied the E-jet3D printing technology to prepare implant drug loaded scaffolds in order to verify their effect of anti-cancer.First,we introduce a novel method for rapid preparation of anti-cancer magnetocaloric PCL/Fe₃O₄ mats capable of high-performance hyperthermia using E-jet 3D printing technology.Magnetic hyperthermia has been rapidly developed as a potential cancer treatment in recent years.Artificially induced hyperthermia close to a tumor can raise the temperature to 45?causing tumor cell death.Our 3D printed scaffolds not only maintained the heating efficiency of traditional techniques for magnetic hyperthermia but also prolonged the effective therapy in vivo.When Fe₃O₄nanoparticles?NPs?were used in mats at a concentration of 6 mmol/L,0.07 g PCL/Fe₃O₄ mats were able to increase the temperature peripherally to 45?under an alternating magnetic field?AMF?within 45 min.Moreover,the reproducibility experiment indicated that the maximum temperature was achieved following repeated heating and cooling cycles.Cell toxicity tests showed a high cell death rate during one treatment cycle.In vivo experiments indicated clear signs of tumor growth inhibitory and prolonged survival time of tumor-bearing mice after 4 weeks of treatment.The present magnetic mats may be a potential candidate for an efficiency heat-generating substrate for localized hyperthermia cancer therapy.Then,we printed drug loaded PLGA scaffolds as carriers for controlled release over prolonged periods of time.PLGA-based implants are very popular as advanced drug delivery systems.In this experiment,we use PLGA to wrap two drugs including 5Fu and NVP-BEZ235 to verify their feasibility of anti-breast cancer.Through experiments we found the pore size of the scaffold could affect the release rate of the drugs,we selected the scaffold with the pore size of 150×150?m which loaded with drugs for in vivo and in vitro research experiments.In vitro results showed after 7days,the therapeutic effect of the drug carrier was consistent with the effect of the free double-drugs.In vivo experiment,the anti-tumor effect of the drug carrier was better than that of the direct injection of drugs,while the total amount of drugs in the drug carrier was far less than the injection.So,we have successfully prepared a drug loaded sustained-release stent for anti-tumor.These two kinds of medical scaffolds proved the feasibility of the E-jet 3D printing technology in interventional therapy.Furthermore,the main advantage of such implantable scaffolds is the local and precise delivery of drugs,which is ideal for the treatment of easily accessible tumors.