Maternal High Salt Diet Altered Adenosine-mediated Vasodilatation Via PKA/BK Channel Pathway In Offspring Rats

Objectives:This study tested the hypothesis that prenatal HS may influence Adenosine-induced vasodilatation via PKA pathway in coronary arteries.Methods:Sprague-Dawley rats were fed with 8%salt diet for gestation,the control was fed with 0.3%salt diet.Coronary arteries from male adult offspring were tested for K⁺channels and Adenosine signal pathways.Results:Adenosine-mediated vasodilatation was reduced in coronary arteries in HS.There was no difference in gene expression of A_2A receptors between the two groups.After pre-treatment with PKA inhibitor,vasodilatation to Adenosine was decreased to a smaller extent in HS than that in control.Forskolin?activator of adenylate cyclase?-mediated vasodilatation was decreased in HS.Iberiotoxin?BK channel inhibtor?attenuated Forskolin-induced vasodilatation in control,not in HS group.Currents of BK channels decreased in coronary artery smooth muscle cells,and PKA-modulated BK channel functions were declined.Protein levels of BK?1 and PKA C-subunits in coronary arteries of HS offspring were reduced.Conclusion:Prenatal HS diets altered Adenosine-mediated coronary artery vasodilatation in the offspring,which was linked to down-regulation of cAMP/PKA/BK channel pathway.