| Objective:To observe the effects of spironolactone on Gal-3 and fibrosis indexes in rats with myocardial infarction.Methods:Male SD rats were performed with permanent coronary artery ligation and divided into three groups after operation:sham operation group(NC,n=6).myocardial infarction group without drugs(MI,n=8),myocardial infarction plus spironolactone group(MI+Spiro,n=8).NC group only open the chest without ligation of the coronary artery.Rats in MI+Spiro group were feeded spironolactone(100mg/kg/d)on the first day after operation.One month later,the expression of Gal-3 and fibrosis in the infarced and non-infarcted areas were detected by RT-qPCR,At the same time,HE and Massson staining were used to observe the morphology and fibrosis of myocardium.We also used the statistical software to analyze the differences of each gene expression.Results:1.Gene expression of Gal-3:Gal-3 expression in infarcted area,MI group was higher than that in NC group(P>0.05).The expression of Gal-3 in MI+Spiro group was lower than that in MI group(P>0.05).In non infarcted area,the expression of Gal-3 in MI group,was higher than that of NC group(P<0.05);the expression of Gal-3 in MI+Spiro group was lower than that in the MI group,and the difference was statistically significant(P<0.05).2.Gene expression of IL-33 : In the infarcted area,the expression level of IL-33 in MI+Spiro group(P<0.05)was higher than that in MI and NC groups.MI group was higher than NC group without significant difference(P>0.05).In the non infarcted area,the levels of IL-33 in MI and MI+Spi group were higher than those in NC group,but there were no significant differences between them.Also,no difference of IL-33 level in MI group and MI+Spi group was found.3.The gene expression of fibrosis and other indexes:(1)In the non infarcted area,type I collagen(P<0.05)and type III collagen(P<0.05)were significantly higher in MI group than that in NC group.Type I collagen in MI+Spiro group was significantly lower than that in MI group(P<0.05).Compared with MI group,the type III collagen of MI+Spiro group was decreased,but not statistically significant.In the non infarcted area,compared with NC group,the expression of I and type III collagen were significantly higher in MI group(P<0.05).In group MI+Spiro,the expression of I and type III collagen were lower than that of MI group,and the differences were statistically significant(P<0.05).(2)TNF-a:In the infarcted area,compared with NC group,the level of inflammatory marker TNF-a in MI group was higher than that in NC group(P<0.05).The level of TNF-a was decreased in MI+Spiro group compared with MI group(P>0.05).In non infarcted area,the TNF-a of MI group was higher than that of NC group,but there was no statistical significance.MI+Spiro group was slightly lower than that of MI group,and with no significant difference.(3)MMP9/TIMP-1:In non infarcted area,the level of MMP9/TIMP-1 in MI+Spiro group was significantly lower than that in MI group(P<0.05).Conclusions: 1.Spironolactone can down regulate the expression of Gal-3 and increase the expression of IL-33 after myocardial infarction.2.Spironolactone may play a role in cardiac remodeling after myocardial infarction by Gal-3pathway.3.Spironolactone improves myocardial fibrosis and inflammation after MI. |
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