The Role Of Phosphorylated Myosin Light Chain And Related Molecules In Smooth Muscle Of Common Bile Duct In Children With Pancreaticobiliary Maljunction

Objective:The aim of the present study was to observe the expression of MYPT1,PP1C,CPI-17 and pMLC20 in smooth muscle of common bile duct in children with pancreaticobiliary maljunction.Method:The common bile duct(CBD)specimens from 12 PBM pediatric patients were compared with normal gallbladder tissues from 10 adults serving as control.The MYPT1,PP1C,CPI-17 and MLC20 protein expression in the two tissues were determined by Western Blot analysis.Results:Western Blot results showed that the expression level of MYPT1,CPI-17,MLC20 protein in PBM group was significantly decreased compared with control group(P<0.01);but the expression level of pMYPT1,pCPI-17,pMLC20 and PP1C protein in PBM group was significantly increased compared with control group(P<0.01).Conclusion:In PBM pediatric patients,the expression of MYPT1 decreased and the expression of pMYPTl was increased,which caused the MLC20 dephosphorylation decreased,and under synergistic action of pCPI-17,increased phosphorylation level of MLC20.Which may lead to the compensatorily enhanced contractile ability of their CBD smooth muscle.